Short coherence length, doppler velocimetry system

• US 5,549,114 A
• Filed: 10/23/1995
• Issued: 08/27/1996
• Est. Priority Date: 10/19/1994
• Status: Expired due to Term

• Current Assignee: Carl Zeiss Inc (Carl-Zeiss-Stiftung)
• Original Assignee: Carl Zeiss Inc (Carl-Zeiss-Stiftung)
• Inventors: Petersen, Christopher L., Hellmuth, Thomas
• Primary Examiner(s): Sykes, Angela D.
• Assistant Examiner(s): WINAKUR, ERIC FRANK
• Application Number:
  US08/546,765
• Time in Patent Office: 309 Days
• Field of Search: 128/664, 128/665, 128/666, 128/667, 128/691, 128/745, 356/28, 356/28.5
• US Class Current: 600/504
• CPC Class Codes: A61B 3/1233 for measuring blood flow, e.g. at the retina

Abstract

Apparatus for measuring the speed of blood in blood vessels in biological samples, for example, in retinal blood vessels, which apparatus includes: (a) a source of a beam of radiation having a principal wavelength, which radiation is substantially spatially coherent and has a temporal coherence length which is less than 1 picosecond; (b) a beam splitter for splitting the beam into a sample beam and reference beam; (c) optical apparatus for directing the sample beam to an area within the biological sample; (d) a reflector for reflecting the reference beam; (e) a detector for detecting an interference between the sample beam reflected from the area and the reflected reference beam and for generating an interference signal; (f) apparatus for altering an optical path length of the reference beam from the beam splitter to the detector at an alteration velocity; and (g) an analyzer for analyzing the interference signal to determine the speed of the blood in the area from a shift of a central frequency of a frequency spectrum of the interference signal from a frequency determined from the alteration velocity and the principal wavelength.

Claims

1. Apparatus for measuring a profile of speed of blood, wherein the speed is parallel or antiparallel to the direction of a sample beam, the profile being across a blood vessel in a biological sample, in particular, in a retinal blood vessel, which apparatus comprises:

a source of a beam of radiation having a first principal wavelength, which beam of radiation is substantially spatially coherent and has a temporal coherence length which is less than 1 picosecond;

means for splitting the beam into the sample beam and a reference beam;

means for reflecting the reference beam which comprises at least one reflective surface;

means for altering an optical path length of the reference beam from the splitting means to a detector means at an alteration velocity, wherein the means for altering comprises means for translating the at least one reflective surface;

an interferometer means which includes a long coherence length radiation source having a second principal wavelength and the at least one reflective surface, the interferometer means producing an interferometer output;

means for directing the sample beam within the biological sample to pass through the blood vessel;

wherein the detector means comprises means;

(a) for detecting interferences between reflections of the sample beam from matter in the sample including matter situated across the blood vessel and reflections of the reference beam from the reflecting means;

(b) for generating an interference signal; and

(c) for detecting the interferometer output and for transmitting an output interferometer signal to analyzer means;

wherein the analyzer means comprises means, in response to the output interferometer signal, for determining the alteration velocity from a central frequency of a frequency spectrum of the output interferometer signal and the second principal wavelength; and

the analyzer means further comprises means for analyzing the interference signal to determine the profile of the speed of the blood from an analysis of frequency spectra of time segments of the interference signal, the alteration velocity in the time segments, and the first principal wavelength.

2. The apparatus of claim 1 wherein the analyzer means comprises means for determining, within a time segment, the speed of blood in a portion of the blood vessel from a central frequency of a frequency spectrum of the interference signal in the time segment, the alteration velocity in the time segment, and the first principal wavelength.

3. The apparatus of claim 2 wherein the analyzer means further comprises means for determining, within the time segment, the speed of blood in the portion of the blood vessel from a width of the frequency spectrum of the interference signal in the time segment, the alteration velocity, the first principal wavelength, and the temporal coherence length.

4. The apparatus of claim 3 wherein the analyzer means further comprises means for identifying the time segment which includes means for detecting maxima of an envelope of the interference signal.

5. The apparatus of claim 4 wherein the means for determining further comprises means for determining the profile of speed of blood from the interference signal generated during a single pass of the sample beam through the blood vessel.

6. The apparatus of claim 4 wherein means for determining further comprises means for determining the profile of speed of blood from interference signals generated during a multiplicity of passes of the sample beam through the blood vessel.

7. The apparatus of claim 1 wherein the source of the beam of radiation is a superluminescent light emitting diode, wherein the beam of radiation is focused through a pinhole aperture.

8. The apparatus of claim 1 wherein the source of the beam of radiation is a superluminescent light emitting diode, wherein the beam of radiation is coupled into a single mode optical fiber.

9. The apparatus of claim 1 wherein the long coherence length source is a laser.

10. The apparatus of claim 1 wherein the beam is coupled into an optical fiber and at least one portion of a path of travel within the apparatus of the sample beam and the reflections of the sample beam is comprised of an optical fiber and at least one portion of a path of travel within the apparatus of the reference beam is comprised of an optical fiber.

11. The apparatus of claim 10 wherein the means for reflecting comprises means for movement of the at least one reflective surface in fixed increments and the means for altering further comprises one or more acousto-optic modulators attached to the optical fiber in the path of travel of the reference beam.

12. The apparatus of claim 10 wherein the means for reflecting comprises means for movement of the at least one reflective surface in fixed increments and the means for altering further comprises one or more piezo-electric transducers attached to the optical fiber in the path of travel of the reference beam.

13. The apparatus of claim 1 wherein a length of the time segments is proportional to the temporal coherence length divided by the alteration velocity.