Methods for production of an optical assay device

• US 5,550,063 A
• Filed: 06/10/1993
• Issued: 08/27/1996
• Est. Priority Date: 02/11/1991
• Status: Expired due to Term

• Current Assignee: Inverness Medical-Biostar, Inc. (Abbott Laboratories)
• Original Assignee: BioStar, Inc. (Thermo Fisher Scientific Incorporated)
• Inventors: Bogart, Gregory R.
• Primary Examiner(s): Saunders, David
• Assistant Examiner(s): CHIN, CHRISTOPHER L
• Application Number:
  US08/076,347
• Time in Patent Office: 1,174 Days
• Field of Search: 427/162, 427/164, 427/165, 427/240, 427/241, 427/414, 427/419.1, 427/419.2, 427/419.3, 427/419.5, 427/419.7, 422/57, 422/55, 422/82.05, 435/4, 435/808, 436/518, 436/524, 436/525, 436/527, 436/528, 436/531, 436/532, 436/805
• US Class Current: 436/518
• CPC Class Codes: G01N 21/211 Ellipsometry optical thickness measurement G01B11/06
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus
• CPC Class Codes: Y10S 436/805 Optical property

Abstract

Method for producing an optical assay device having a substrate and one or more optical layers, an attachment layer and a receptive layer, including the step of spin coating an anti-reflective layer or an attachment layer.

Claims

1. A method for producing an optical assay device which produces a detectable signal caused by light interference effects of said device comprising a substrate and one or more optical layers, an attachment layer and a receptive layer, comprising the steps of:

providing a substrate,

spin coating an anti-reflective layer onto said substrate,

providing an attachment layer, formed from one or more members selected from the group consisting of non-linear branched polymeric siloxanes, film forming latexes, and dendrimers, on said anti-reflective layer, and

providing a receptive layer on said attachment layer.

2. The method of claim 1 wherein said anti-reflective layer is formed from one or more members selected from the group consisting of:

titanium dioxide, diamond, polysilizanes, aluminum alkyloxides, titanates, and T-resin siloxanes.

3. The method of claim 2, wherein said anti-reflective layer has a thickness between 250 and 550 Å

.

4. The method of claim 2, wherein said anti-reflective layer is amorphous.

5. The method of claim 1, wherein said attachment layer has a thickness between 25 and 250 Å

.

6. A method for producing an optical assay device which exhibits a first color in response to light impinging upon an optical surface, and exhibiting a second color comprising a combination of wavelengths of light different from said first color in response to said light when an analyte is present on said surface, comprising a substrate, an anti-reflective layer, an attachment layer and a receptive layer comprising the steps of;

providing said substrate,

spin coating onto said substrate said anti-reflective layer,

providing said attachment layer, formed from one or more members selected from the group consisting of non-linear branched polymeric siloxanes, film forming latexes, and dendrimers, on said anti-reflective layer, and

providing said receptive layer on said attachment layer.

7. The method of claim 6, comprising the step of spin coating said attachment layer on said anti-reflective layer of said optical device.

8. The method of claim 7, wherein said attachment layer has a thickness between 25 and 250 Å

.

9. The method of claim 6, wherein said anti-reflective layer is formed from one or more members selected from the group consisting of:

titanium dioxide, diamond, polysilizanes, aluminum alkyloxides, titanates, and T-resin siloxanes.

10. A method for producing an optical assay device which exhibits a first color in response to light impinging upon an optical surface, and exhibiting a second color comprising a combination of wavelengths of light different from said first color in response to said light when an analyte is present on said surface, comprising a substrate, an anti-reflective layer, an attachment layer and a receptive layer comprising the steps of;

providing said substrate,

providing said anti-reflective layer on said substrate,

spin coating said attachment layer, formed from one or more members selected from the group consisting of non-linear branched polymeric siloxanes, film forming latexes, and dendrimers, on said anti-reflective layer, and

providing said receptive layer on said attachment layer.

14. The method of claims 10, 11, 12, or 13 wherein said attachment layer has a thickness between 25 and 250 Å

.

11. A method for producing an optical assay device which exhibits a first color in response to light impinging upon an optical surface, and exhibiting a second color comprising an intensity of at least one said wavelength of light different from said first color, in response to said light when an analyte is present on said surface, comprising a substrate, an attachment layer and a receptive layer comprising the steps of;

providing said substrate,

spin coating said attachment layer, formed from one or more members selected from the group consisting of non-linear branched polymeric siloxanes, film forming latexes, and dendrimers, on said substrate layer, and

providing said receptive layer on said attachment layer.

12. A method for producing an optical assay device which produces a detectable signal caused by light interference effects of said device comprising a substrate and one or more optical layers, an attachment layer and a receptive layer, comprising the steps of:

providing a substrate,

providing an anti-reflective layer onto said substrate,

spin coating said attachment layer, formed from one or more members selected from the group consisting of non-linear branched polymeric siloxanes, film forming latexes, and dendrimers, on said anti-reflective layer, and

providing said receptive layer on said attachment layer.

13. A method for producing an optical assay device which produces a signal that is detectable by a thin film measurement technique comprising a substrate, an attachment layer and a receptive layer, comprising the steps of:

providing said substrate,

spin coating said attachment layer, formed from one or more members selected from the group consisting of non-linear branched polymeric siloxanes, film forming latexes, and dendrimers, on said substrate, and

providing said receptive layer on said attachment layer.