Method and apparatus for analytical determination of glucose in a biological matrix
First Claim
1. A method for determination of glucose concentration in a biological matrix, said method comprising the steps of:
- performing at least two detection measurements, each detection measurement comprising the further steps of irradiating light at a first irradiation site as primary light into the biological matrix through a boundary surface thereof, said light being propagated along a light path within the biological matrix, and measuring an intensity of the light as the light emerges from the biological matrix as secondary light through a first detection site of the boundary surface thereof;
wherein at least one of said at least two detection measurements is a spatially resolved measurement of multiply scattered light, wherein the primary light is irradiated into the biological matrix at the first irradiation site, the intensity of the secondary light emerging from the biological matrix at the first detection site is measured, and wherein the first detection site is located relative to the first irradiation site such that light which was multiply scattered by scattering centers in the biological matrix is detected, and wherein the light paths of said at least two detection measurements are different, andderiving the glucose concentrations from the dependence of the intensity of the secondary light from the relative position of the first irradiation site and the first detection site in said at least two detection measurements.
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Abstract
A method for determining glucose concentration in a biological matrix includes the steps of performing at least two detection measurements. In each detection measurement light is irradiated at a defined irradiation site as primary light into the biological matrix, through a boundary surface thereof. The light is propagated along a light path within the biological matrix. An intensity of the light is measured as the light emerges as secondary light through a defined detection site of the boundary surface. At least one of the detection measurements is a spatially resolved measurement of multiply scattered light. The detection site is located relative to the irradiation site such that light which was multiply scattered at scattering centers in the biological matrix is detected. The light paths of the at least two detection measurements within the biological matrix are different. Glucose concentration is then derived from the dependence of the intensity of the secondary light from the relative position of the irradiation site and the detection site.
317 Citations
101 Claims
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1. A method for determination of glucose concentration in a biological matrix, said method comprising the steps of:
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performing at least two detection measurements, each detection measurement comprising the further steps of irradiating light at a first irradiation site as primary light into the biological matrix through a boundary surface thereof, said light being propagated along a light path within the biological matrix, and measuring an intensity of the light as the light emerges from the biological matrix as secondary light through a first detection site of the boundary surface thereof; wherein at least one of said at least two detection measurements is a spatially resolved measurement of multiply scattered light, wherein the primary light is irradiated into the biological matrix at the first irradiation site, the intensity of the secondary light emerging from the biological matrix at the first detection site is measured, and wherein the first detection site is located relative to the first irradiation site such that light which was multiply scattered by scattering centers in the biological matrix is detected, and wherein the light paths of said at least two detection measurements are different, and deriving the glucose concentrations from the dependence of the intensity of the secondary light from the relative position of the first irradiation site and the first detection site in said at least two detection measurements. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53)
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54. A method for spectral analytical determination of an analyte in a biological matrix, said method comprising the steps of:
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performing at least two detection measurements, each of said at least two detection measurements being for measurement of spectral dependence of absorption of the analyte, said detection measurements being at at least two different wavelengths, each detection measurement comprising the further steps of irradiating light at a first irradiation site as a primary light into the biological matrix through a boundary surface thereof, said light being propagated along a light path within the biological matrix, and measuring an intensity of the light as the light emerges from the biological matrix as secondary light through a first detection site of the boundary surface thereof; performing an evaluation step wherein the analyte concentration is derived from the measured intensities of the detection measurements by means of an evaluation algorithm and a calibration; wherein at least one of said at least two detection measurements is a spatially resolved measurement of multiply scattered light wherein the primary light is irradiated into the biological matrix by the first irradiation site, the intensity of the secondary light emerging from the biological matrix at the first detection site is measured, and wherein the first detection site is located relative to the first irradiation site such that light which was multiply scattered by scattering centers in the biological matrix is detected, and wherein the light paths of said at least two detection measurements within the biological matrix are different, and deriving a correction value for the evaluation algorithm for correction due to changes in an optical path length in said at least two detection measurements, said correction value being derived from the dependence of the intensity of the secondary light on the relative position of the first irradiation site and the first detection site. - View Dependent Claims (55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101)
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Specification