Automatic sample container handling centrifuge and a rotor for use therein

• US 5,551,941 A
• Filed: 06/05/1995
• Issued: 09/03/1996
• Est. Priority Date: 10/14/1993
• Status: Expired

• Current Assignee: Siemens Healthcare Diagnostics Incorporated (Siemens AG)
• Original Assignee: E. I. du Pont de Nemours and Company (Corteva, Inc.)
• Inventors: Howell, Gary W.
• Primary Examiner(s): Scherbel, David
• Assistant Examiner(s): Till, Terrence R.
• Application Number:
  US08/461,466
• Time in Patent Office: 456 Days
• Field of Search: 221/113, 221/82, 221/86, 221/89, 422/101, 422/72, 436/45, 436/177, 494/16, 494/21, 494/31
• US Class Current: 494/16
• CPC Class Codes: B04B 2007/025 Lids for laboratory centrifuge rotors
• CPC Class Codes: B04B 2011/046 Loading, unloading, manipulating sample containers
• CPC Class Codes: B04B 5/0414 comprising test tubes
• CPC Class Codes: Y10T 436/111666 Utilizing a centrifuge or compartmented rotor

Abstract

A centrifuge instrument and a rotor therefor which is loaded with sample containers automatically by gravity through an opening in a cover located over a core and, subsequent to centrifugation, is unloaded automatically, again assisted by gravity, through an opening in a floor disposed beneath the core. A sample container loading arrangement is provided to hold a plurality of sample containers and to individually present sample containers to the rotor.

Claims

1. A centrifuge rotor for rotating a sample container about an axis of rotation, the rotor comprising:

a core having at least one container-receiving cavity extending completely therethrough, the core comprising;

a generally cylindrical central portion having a core mounting aperture therethrough, and

a generally frustoconical radially outward portion, the container-receiving cavity being disposed in the generally frustoconical radially outward portion;

a floor, the floor and the core being positioned relative to each other so that, unless inhibited by the floor a container receivable within the cavity in the core would drop by gravity therefrom,

the floor and the core being movable together as a unit and also being movable with respect to each other from a closed position to an open position,

in the closed position the floor at least partially closes the cavity in the core to inhibit a container receivable within the core from dropping from the core in response to gravity, while in the open position the container receivable within the cavity responds to gravity to drop from the core;

a first latch system for selectably latching the floor and the core to maintain the core and the floor in the closed position.

3. The centrifuge rotor of claim 1 wherein the floor comprises:

a generally cylindrical central portion having a floor mounting aperture therethrough, and a generally frustoconical radially outward skirt portion, the skirt portion having the unloading port formed therethrough,

the cylindrical portion of the floor and the cylindrical portion of the core being arranged such that the mounting apertures in the floor and in the core register axially with each other,

wherein the first latch system is disposed in the cylindrical portions of the floor and the core.

4. The centrifuge rotor of claim 3 wherein the first latch system comprises:

a recess formed in the central portion of the core,

a latching opening in the central portion of the floor, the recess and the latching opening registering with each other,

a latching member received in the recess such that a portion thereof extends into and is received by the latching opening in the floor, thereby to latch the floor to the core; and

a plunger extensible to engage the portion of the latching member received by the latching opening in the floor and to urge the portion of the latching member therefrom, thereby to unlatch the floor from the core.

5. The centrifuge rotor of claim 1 wherein the container-receiving cavity in the core has radially inner and radially outer boundary walls, the boundary walls being disposed parallel to the axis of rotation.

6. The centrifuge rotor of claim 1 wherein the core has a surface thereon, the surface of the core being subdivisible into a plurality of segments, the surface of some of the plurality of segments being interrupted by a sample container-receiving cavity that extends through the core while the surface of others of the segments is uninterrupted, some of the cavities being angularly spaced from an adjacent cavity by a first angular distance while others of the cavities are angularly spaced from an adjacent cavity by a second angular distance, the greater angular distance encompassing an uninterrupted surface of a segment of the core.

7. The centrifuge rotor of claim 6 wherein the core includes at least two uninterrupted segments that are diametrically opposed to each other.

8. The centrifuge rotor of claim 1 wherein the cover comprises:

a generally cylindrical central portion having a cover mounting aperture therein; and

a generally frustoconical radially outward skirt portion, an annular lip depending from the skirt portion, the skirt portion having the loading port formed therein.

9. The centrifuge rotor of claim 8 wherein the core comprises:

a generally cylindrical central portion having a core mounting aperture therethrough; and

a generally frustoconical radially outward portion, the container-receiving cavity being disposed in the generally frustoconical radially outward portion,

the cylindrical central portion of the cover and the cylindrical central portion of the core being arranged such that the cover mounting aperture registers axially with the core mounting aperture, and wherein

the frustoconical radially outward portion of the core and the lip on the cover are confrontationally arranged, the second latch system being disposed in the confrontationally arranged portions of the cover and the core.

10. The centrifuge rotor of claim 9 wherein the second latch system comprises:

a second recess formed in the frustoconical portion of the core,

a second latching opening in the lip portion of the cover, the second recess and the second latching opening registering with each other,

a second latching member received in the second recess such that a portion thereof extends into and is received by the second latching opening, thereby to latch the cover to the core; and

a plunger extensible to engage the potion of the second latching member received by the second latching opening and to urge the portion of the latching member therefrom

thereby to unlatch the cover from the core.

22. The centrifuge rotor of claim 1 wherein the container-receiving cavity has a mouth that opens in the central portion of the core, the cavity extending into the generally frustoconical radially outward portion.

23. The centrifuge rotor of claim 22 wherein the rotor further comprises a cover, the cover comprising:

a generally cylindrical central portion having a mounting aperture and a plurality of loading ports formed therein;

a generally frustoconical radially outward skirt portion; and

wherein each of the plurality of loading ports is axially registered with the container-receiving cavities in the core.

2. The centrifuge rotor of claim 2 further comprising:

a cover having a loading port therethrough, the cover and the core being movable with respect to each other to a loading position in which the loading port registers with the cavity in the core; and

a second latch system for selectably latching the cover and the core.

11. A centrifuge instrument for rotating a sample container about an axis of rotation, the instrument comprising:

a rotor, the rotor comprising;

a core having at least one container-receiving cavity extending completely therethrough;

a floor, the floor and the core being positioned relative to each other so that, unless inhibited by the floor a container receivable within the cavity in the core would drop by gravity therefrom,

the floor and the core being movable together as a unit and also being movable with respect to each other from a closed position to an open position,

in the closed position the floor at least partially closes the cavity in the core to inhibit a container receivable within the core from dropping from the core in response to gravity, while in the open position the container receivable within the cavity responds to gravity to drop from the core;

a first latch system for selectably latching the floor and the core in a latched state and in an unlatched state, in the latched state the floor and the core occupy the closed position and are movable together as a unit,

in the unlatched state the core is movable with respect to the floor while the core is maintained in a predetermined angular location with respect to the axis of rotation; and

a motive source connected to the core for rotating the core and the floor as a unit when the latch is in the latched state and for rotating the core with respect to the floor when the latch is in the unlatched state.

12. The instrument of claim 11 wherein the rotor further comprises:

a cover having a loading port therein, the cover and the core being movable with respect to each other;

a second latch system for selectably latching the cover and the core in a latched state and in an unlatched state, and in the latched state the cover and the the core are movable together as a unit, in the unlatched state the cover is maintained in a predetermined angular loading location with respect to the axis of rotation and the core is movable with respect thereto to bring the cavity into registration beneath the loading port to permit a container to drop by gravity into the core through the loading port;

the motive source being operative to move the core with respect to the cover when the second latch is in the unlatched state.

13. The instrument of claim 12 wherein the instrument further comprises:

a tray having a loading slot therein, the slot being located at the same predetermined angular loading location with respect to the axis of rotation as is occupied by the loading port in the cover;

so that, with the second latch in the unlatched state, as the core is moved by the motive source with respect to the cover the cavity in the core is brought into registration beneath both the loading slot in the tray and the loading port in the cover,

thereby to permit a container to drop by gravity into the core through the registered loading slot in the tray and the loading port in the cover.

14. The instrument of claim 13 wherein the instrument further comprises:

a loading wheel mounted coaxially above the tray, the wheel having a plurality of cavities therein, each cavity being sized to receive a sample container; and

means for rotating the loading wheel and the core to bring a container disposed in a cavity in the wheel into registration with the slot in the tray.

15. The instrument of claim 14 wherein the instrument further comprises:

a magazines member disposed above the loading wheel, the magazine member having at least one magazine therein for generating a singulated stream of sample containers and for sequentially guiding each container in the stream into cavities in the loading wheel as the rotating means rotates the loading wheel.

16. The instrument of claim 11 further comprising an unloading chute, the chute being located at the same predetermined angular unloading location with respect to the axis of rotation as is occupied by the unloading port in the floor, the chute being positioned to receive a sample container dropping by gravity through the unloading port.

17. The instrument of claim 11 wherein the rotor comprises:

a generally cylindrical central portion having a core mounting aperture therethrough, and

a generally frustoconical radially outer portion,

the container-receiving cavity having a mouth that opens in the central portion of the core, the cavity extending into the generally frustoconical radially outward portion

and wherein the rotor further comprises

a cover having a plurality of loading ports formed therein, each loading port being registered with the mouth of one container-receiving cavity in the core, the cover being secured to the core.

18. The instrument of claim 17 wherein the instrument further comprises:

a tray having a receiving trough therein, the trough having an opening, the opening being axially registered with one of the loading ports in the cover,

whereby a container drops by gravity into the core through the registered opening in the trough and the corresponding loading port in the cover.

19. The instrument of claim 18 wherein the instrument further comprises:

a magazine member disposed above the tray, the magazine member having at least one magazine therein for generating a singulated stream of sample containers; and

a loading member for loading a sample container from the magazine member into the receiving trough.

20. The instrument of claim 19 wherein the loading member comprises:

a motor; and

a loading rod acting in cooperation with the motor, the loading rod urging the sample container from the magazine into the receiving trough.

21. In a sample test analyzer having

a sample analysis device,

a centrifuge instrument having a rotor for exposing a sample in a sample container to a centrifugal force field, and,

a transport for transporting small containers having a sample therein from the centrifuge instrument to the sample analysis device,

an improved rotor comprising:

a core having at least one container-receiving cavity extending completely therethrough; and

a floor, the floor and the core being positioned relative to each other so that, unless inhibited by the floor a container receivable within the cavity in the core would drop by gravity therefrom,

the floor and the core being movable together as a unit and also being movable with respect to each other from a closed position to an open position,

in the closed position the floor at least partially closes the cavity in the core to inhibit a container receivable within the core from dropping from the core in response to gravity, while in the open position the container receivable within

the cavity responds to gravity to drop from the core.