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Liposome for entrapping gene, liposomal preparation and process for the manufacture of the preparation

  • US 5,552,157 A
  • Filed: 03/28/1995
  • Issued: 09/03/1996
  • Est. Priority Date: 08/27/1990
  • Status: Expired due to Term
First Claim
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1. A process for producing a deoxyribonucleic acid entrapping multilamellar liposome, comprising the following steps:

  • dissolving lipids of N-(α

    -trimethylammonioacetyl)-didodecyl-D-glutamate chloride, dilauroylphosphatdylcholine and dioleophosphatidylethanolamine in a molar ratio of 1;

    2;

    2, in a solvent suitable for forming a lipid film,removing the solvent with an evaporator to form a lipid film,adding a deoxyribonucleic acid solution to said lipid film to form a mixture, andshaking said mixture with a vortex mixer to form a deoxyribonucleic acid entrapping multilamellar liposome.

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