Apparatus and process for simplified measurement
First Claim
1. A simplified measuring apparatus for use in a binding assay to determine the presence or amount of an analyte in a fluid test sample through the use of a label capable of producing a detectable response, said apparatus comprising:
- (a) a porous body having releasably attached thereto an agent soluble in said test sample;
(b) a liquid permeable porous reaction membrane disposed below said porous body having defined thereon at least one reaction area, said reaction area having immobilized thereon an affinity substance capable of directly or indirectly capturing the analyte or an agent soluble in said test sample to thereby produce the detectable response;
(c) an absorption member disposed below said liquid permeable porous membrane having an opening being positioned below the reaction area of said liquid permeable porous membrane to define a reaction solution storing space within the opening, said absorption member being, arranged so as to contact only a peripheral area of said liquid permeable porous membrane via an intervening liquid impermeable sheet;
(d) a liquid impermeable transparent cover disposed below said opening of said absorption member; and
(e) a liquid impermeable case accommodating said members (a), (b), (c) and (d) having a top surface adjacent said porous body and a bottom surface adjacent said transparent cover, said top surface having defined therein a top opening for introducing said fluid test sample, and said bottom surface having defined therein a bottom opening for observation of said detectable response.
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Accused Products
Abstract
A simplified measuring apparatus for use in the qualitative or quantitative measurement of substances to be assayed in test samples, such as proteins, antibodies and the like, in a small amount of test samples by a simple and easy operation without requiring a B/F separation step comprises: (a) a liquid permeable porous reaction membrane whose surface having at least one reaction area to which an affinity substance capable of directly or indirectly capturing a substance to be assayed or a soluble agent is immobilized; (b) a porous body arranged on the upper part of the porous reaction membrane, to which a soluble agent capable of being solubilized by the addition of a test sample is adhered in a releasable manner; (c) an absorption member arranged on the lower part of the porous reaction membrane, which contacts with a periphery, excluding the reaction area, of the porous reaction membrane via a liquid non-permeable sheet; (d) a liquid non-permeable transparent cover arranged on the lower part of the absorption member; and (e) a liquid non-permeable case which encloses the foregoing members (a) to (d), having an opening for use in test sample introduction on its top surface and another opening for observation on its under surface.
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Citations
19 Claims
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1. A simplified measuring apparatus for use in a binding assay to determine the presence or amount of an analyte in a fluid test sample through the use of a label capable of producing a detectable response, said apparatus comprising:
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(a) a porous body having releasably attached thereto an agent soluble in said test sample; (b) a liquid permeable porous reaction membrane disposed below said porous body having defined thereon at least one reaction area, said reaction area having immobilized thereon an affinity substance capable of directly or indirectly capturing the analyte or an agent soluble in said test sample to thereby produce the detectable response; (c) an absorption member disposed below said liquid permeable porous membrane having an opening being positioned below the reaction area of said liquid permeable porous membrane to define a reaction solution storing space within the opening, said absorption member being, arranged so as to contact only a peripheral area of said liquid permeable porous membrane via an intervening liquid impermeable sheet; (d) a liquid impermeable transparent cover disposed below said opening of said absorption member; and (e) a liquid impermeable case accommodating said members (a), (b), (c) and (d) having a top surface adjacent said porous body and a bottom surface adjacent said transparent cover, said top surface having defined therein a top opening for introducing said fluid test sample, and said bottom surface having defined therein a bottom opening for observation of said detectable response. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification