DNA base sequencer

• US 5,552,322 A
• Filed: 09/28/1994
• Issued: 09/03/1996
• Est. Priority Date: 09/28/1993
• Status: Expired due to Fees

• Current Assignee: Hitachi High-Tech Electronics Engineering Co., Ltd. (Hitachi, Ltd.)
• Original Assignee: Hitachi High-Tech Electronics Engineering Co., Ltd. (Hitachi, Ltd.)
• Inventors: Nemoto, Ryozi, Mishina, Yoshinori
• Primary Examiner(s): Redding, David A.
• Application Number:
  US08/313,912
• Time in Patent Office: 706 Days
• Field of Search: 204/182.8, 204/299 R, 356/344, 422/82.08, 422/131, 422/187, 435/291, 935/86, 935/88
• US Class Current: 435/287.2
• CPC Class Codes: G01N 27/44721 by optical means
• CPC Class Codes: G01N 27/44782 of a plurality of samples

Abstract

The improved DNA base sequencer comprises a flat plate type gel electrophoretic means that has a multiple of tracks for electrophoresing DNA fragments and which is held in a vertical position, a light exciting laser light applying means that applies laser light to the respective tracks in said electrophoretic means from one lateral side thereof in such a way that it crosses said tracks at right angles, and a fluorescence detecting means that detects the fluorescence as generated from the DNA fragments illuminated with the laser light and which converts the detected fluorescence to an electric signal, and it is characterized in that the fluorescence detecting means comprises an index-distributed lens array, a filter and a solid-state imaging device such as a CCD line sensor. This apparatus uses light-receiving optics that does not include a large and expensive optical device such as an image intensifier and which yet is capable of efficient fluorescence detection without "smiling" and other adverse effects.

Claims

1. In a DNA base sequencer comprising a flat plate type gel electrophoretic means that has a multiple of tracks for electrophoresing DNA fragments and which is held in a vertical position, a light exciting laser light applying means that applies laser light to the respective tracks in said electrophoretic means from one lateral side thereof in such a way that it crosses said tracks at right angles, and a fluorescence detecting means that detects the fluorescence as generated from the DNA fragments illuminated with the laser light and which converts the detected fluorescence to an electric signal, the improvement wherein said fluorescence detecting means comprises an index-distributed lens array, a filter and a solid-state imaging device.

2. A DNA base sequencer according to claim 1 wherein said index-distributed lens array consists of an upper and a lower row of index-distributed lenses.

3. A DNA base sequencer according to claim 1 wherein said solid-state imaging device is a CCD line sensor.

4. A DNA base sequencer according to claim 1 wherein said fluorescence detecting means comprises, in order from the electrophoretic means side, the index-distributed lens array, the filter and the CCD line sensor.

5. A DNA base sequencer according to claim 4 wherein said CCD line sensor is cooled with Peltier devices.