Multiple oligonucleotide containing oligomers and the cleanable linkers used in their preparation
First Claim
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1. A compound of formula (3):
- ##STR35## wherein one or both of A1 and A2 is a divalent group of the formula (a);
##STR36## in which R1 and R2 are each independently H, optionally substituted alkyl, optionally substituted alkoxy, optionally substituted aryloxy, or an electron withdrawing group;
Y is CH2 ;
CH2 CH2, NH, S or O;
E is an organic spacer group; and
any remaining group represented by A1 or A2 is of the formula (b);
##STR37## wherein;
n has a value of from 1 to 5;
the carbon atom marked with an asterisk is attached to an oxygen atom shown in Formula (3); and
each R3 independently represents H or optionally substituted alkyl;
Z1 is an acid labile protecting group; and
--O--PA is a phosphoramidite group, a phosphate ester group or a H-phosphonate group.
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Abstract
A method for the synthesis of a plurality of oligonucleotides comprising the steps of
(i) forming a first oligonucleotide on a first cleavable link attached to a solid support;
(ii) attaching to the first oligonucleotide a cleavable linker moiety;
(iii) forming a second oligonucleotide on the cleavable linker moiety; and
(iv) cleaving the first cleavable link and the cleavable linker moiety to give a plurality of oligonucleotides; wherein the cleavable linker moiety is of the Formula (1): ##STR1## in which A1, A2 and E are as defined herein, and novel compounds which may be used in the operation of the method.
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Citations
14 Claims
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1. A compound of formula (3):
- ##STR35## wherein one or both of A1 and A2 is a divalent group of the formula (a);
##STR36## in which R1 and R2 are each independently H, optionally substituted alkyl, optionally substituted alkoxy, optionally substituted aryloxy, or an electron withdrawing group;
Y is CH2 ;
CH2 CH2, NH, S or O;
E is an organic spacer group; and
any remaining group represented by A1 or A2 is of the formula (b);
##STR37## wherein;
n has a value of from 1 to 5;
the carbon atom marked with an asterisk is attached to an oxygen atom shown in Formula (3); and
each R3 independently represents H or optionally substituted alkyl;
Z1 is an acid labile protecting group; and
--O--PA is a phosphoramidite group, a phosphate ester group or a H-phosphonate group. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
- ##STR35## wherein one or both of A1 and A2 is a divalent group of the formula (a);
Specification