Production of recombinant human interferon-beta.sub.2

• US 5,554,513 A
• Filed: 12/12/1989
• Issued: 09/10/1996
• Est. Priority Date: 11/21/1979
• Status: Expired due to Term

• Current Assignee: Yeda Research and Development Co. Ltd. (Weizmann Institute of Science)
• Original Assignee: Yeda Research and Development Co. Ltd. (Weizmann Institute of Science)
• Inventors: Zilberstein, Asher, Revel, Michel
• Primary Examiner(s): Martinell, James
• Application Number:
  US07/449,447
• Time in Patent Office: 2,464 Days
• Field of Search: 435/69.51, 435/91, 435/172.3, 435/240.2, 435/252.3-252.35, 435/320.1, 536/27, 536/23.52, 935/11, 935/29, 935/34, 935/70-75
• US Class Current: 435/69.51
• CPC Class Codes: A61K 38/215 IFN-beta
• CPC Class Codes: C07K 14/5412 IL-6
• CPC Class Codes: C07K 14/565 IFN-beta

Abstract

Human interferon-beta₂.sbsb.A and interferon-beta₂.sbsb.B are produced in purified form by recombinant DNA techniques. Two separate human genes have been identified which code for the production of IFN-β₂.sbsb.A and IFN-β₂.sbsb.B, respectively. The sequence of IFN-β₂.sbsb.A cDNA is established. These genes and cDNA have been cloned into mammalian cells with an SV40 early promoter sequence and such genomic clones are capable of producing IFN-β₂.sbsb.A and IFN-β₂.sbsb.B. The anti-viral activity of such recombinant IFN-β₂.sbsb.A and IFN-β₂.sbsb.B is demonstrated as well as other biological activity identifying them as human interferons.

Claims

1. A recombinant vector comprising a DNA sequence which codes for human interferon-beta₂.sbsb.A and further comprising a regulatory region positioned in a way that expression of the human interferon beta₂.sbsb.A is possible.

2. A recombinant vector in accordance with claim 1, wherein said regulatory region is an SV40 early promoter sequence.

3. A cell comprising a transformed cell transformed by a recombinant vector in accordance with claim 2, or progeny thereof containing the genetic information imparted by said vector.

4. A cell comprising a transformed cell, transformed by a recombinant vector in accordance with claim 1, or progeny thereof containing the genetic information imparted by said vector.

5. A process for the production of interferon-β

_2A (IFN-β

_2A) comprising culturing cells in accordance with claim 4 under conditions sufficient for the expression of IFN-β

_2A.

6. A recombinant vector in accordance with claim 1 wherein said DNA sequence is a genomic DNA sequence containing the human interferon beta₂.sbsb.A gene.

7. A recombinant vector in accordance with claim 6, consisting essentially of pSVIFA2-I.

8. A recombinant vector in accordance with claim 6, consisting essentially of pSVIFA2-II.

9. A cell comprising a transformed cell transformed by a recombinant vector in accordance with claim 6, or progeny thereof containing the genetic information imparted by said vector.

10. A cell in accordance with claim 9, wherein said cell is a CHO cell.

11. A cell in accordance with claim 9, wherein said cell is an L-TK cell.

12. A cell in accordance with claim 9, wherein said cell is eukaryotic.

13. A cell in accordance with claim 12, wherein said cell is a hamster cell.

14. A process for the production of interferon-beta₂.sbsb.A comprising culturing cells in accordance with claim 12 under conditions sufficient for the expression of interferon-beta₂.sbsb.A.

15. A recombinant vector in accordance with claim 1 wherein said DNA sequence is a cDNA which codes for human interferon-beta₂.sbsb.A.

16. A cell comprising a transformed cell transformed by a recombinant vector in accordance with claim 15, or progeny thereof containing the genetic information imparted by said vector.

17. A cell in accordance with claim 16, comprising a bacterial cell.

18. A process for the production of interferon-beta₂.sbsb.A comprising culturing cells in accordance with claim 17 under conditions sufficient for the expression of interferon-beta₂.sbsb.A.

19. A recombinant vector in accordance with claim 15 wherein said interferon-beta₂.sbsb.A c/DNA is the interferon-beta₂.sbsb.A cDNA as shown in FIG. 1.

20. A recombinant vector in accordance with claim 19, consisting essentially of pSVCIFβ

2.

21. A cell comprising a transformed cell transformed by a recombinant vector in accordance with claim 19, or progeny thereof containing the genetic information imparted by said vector.

22. A non-human cell, containing a human gene consisting essentially of the human interferon-β

_2A gene.

23. A cell in accordance with claim 22, wherein said human interferon-β

_2A gene is the IFA-2 gene.

24. A cell in accordance with claim 22, capable of producing a polypeptide having the amino acid sequence of human interferon-β

_2A.