Device for performing timed assay by electrical resistance change
First Claim
1. A detection unit comprising:
- a support stage for receiving a test article, the test article having spaced-apart electrodes in electrical contact with a reaction zone, wherein the reaction zone on the test article is disposed at a viewing location;
a heater attached to the support stage for heating the test article in the region of the reaction zone;
means attached to the support stage for detecting electrical resistance across the test article;
means attached to the support stage for optically viewing the reaction zone when disposed at the viewing location;
means in communication with the electrical resistance detecting means for initiating a timing cycle when the electrical resistance detecting means detects a lowering of electrical resistance across the test article upon application of a fluid sample having an amount of analyte therein to the test article;
means for actuating the optical viewing means and the electrical resistance detecting means at one or more times during the timing cycle to measure one or more optically detectable signals and electrical resistance values at known times during the timing cycle; and
means for correlating the measured signals and values from the optical viewing means and the electrical resistance detecting means with the known times at which the signals and values are measured so that the amount of analyte initially present in the sample may be determined.
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Accused Products
Abstract
Assays for liquid analytes are performed on a bibulous matrix containing dried reagents which produce a visibly detectable reaction product. Application of liquid sample to the bibulous matrix is detected by measuring a drop in resistance across the matrix. A preferred test article for performing the method includes the matrix and a pair of spaced-apart electrodes in electrical contact with a reaction zone on the matrix. The test article is used in combination with a detection unit having means for probing the electrodes to determine when electrical resistance in the matrix has decreased. The assay methods and apparatus are particularly useful for performing enzyme assays where signal developed as a function of time is critical.
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Citations
12 Claims
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1. A detection unit comprising:
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a support stage for receiving a test article, the test article having spaced-apart electrodes in electrical contact with a reaction zone, wherein the reaction zone on the test article is disposed at a viewing location; a heater attached to the support stage for heating the test article in the region of the reaction zone; means attached to the support stage for detecting electrical resistance across the test article; means attached to the support stage for optically viewing the reaction zone when disposed at the viewing location; means in communication with the electrical resistance detecting means for initiating a timing cycle when the electrical resistance detecting means detects a lowering of electrical resistance across the test article upon application of a fluid sample having an amount of analyte therein to the test article; means for actuating the optical viewing means and the electrical resistance detecting means at one or more times during the timing cycle to measure one or more optically detectable signals and electrical resistance values at known times during the timing cycle; and means for correlating the measured signals and values from the optical viewing means and the electrical resistance detecting means with the known times at which the signals and values are measured so that the amount of analyte initially present in the sample may be determined. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A detection unit for use in combination with a test article comprising:
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(a) a bibulous matrix; (b) one or more dried reagents which are present within the matrix and which, when wetted by a liquid sample, initiate an optically detectable chemical reaction with an analyte present in the sample; (c) a pair of spaced-apart metal strips proximate a sample target location of the matrix, wherein the metal strips are disposed so that application of the liquid sample to the target location will lower electrical resistance between the electrode wherein the detection unit comprises; a support stage for receiving the test article, where a reaction zone on the test article is disposed at a viewing location; a heater attached to the support stage for heating the test article in the region of the reaction zone; means attached to the support stage for detecting electrical resistance across the test article; means attached to the support stage for optically viewing the reaction zone when disposed at the viewing location; means in communication with the electrical resistance means for initiating a timing cycle when the electrical resistance detecting means detects a lowering of electrical resistance between the electrodes upon application the sample to the test article; means for actuating the optical viewing means and the electrical resistance detecting means at one or more times during the timing cycle to measure one or more optically detectable signals and electrical resistance values at known times during the timing cycle; and means for correlating the measured signals and value from the optical viewing means and the electrical resistance detecting means with the known times at which the signals and values are measured so that the amount of analyte initially present in the sample may be determined. - View Dependent Claims (8, 9, 10, 11, 12)
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Specification