DNA sequences derived from papillomavirus HPV-33 genome

• US 5,554,538 A
• Filed: 03/25/1994
• Issued: 09/10/1996
• Est. Priority Date: 03/21/1986
• Status: Expired due to Term

• Current Assignee: Roche Molecular Systems (Roche Holding AG)
• Original Assignee: Institut Pasteur
• Inventors: Cole, Stewart, Streeck, Rolf E.
• Primary Examiner(s): MOSHER, MARY
• Application Number:
  US08/222,569
• Time in Patent Office: 900 Days
• Field of Search: 435/320.1, 435/172.3, 435/5, 536/24.1, 536/24.32, 536/23.72
• US Class Current: 435/320.1
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: A61P 31/12 Antivirals
• CPC Class Codes: A61P 37/04 Immunostimulants
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C12N 2710/20022 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 7/00 Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00
• CPC Class Codes: C12Q 1/708 for papilloma

Abstract

The invention concerns DNA fragments derived from the genomic DNA of HPV-33. These fragments are selected from the group of fragments extending between the nucleotide extremities defined hereafter in relation to the nucleotide-numbering in FIGS. 1a and 1b respectively:

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76-556 543-864 867-2811 2728-3808 3326-3575 3842-4079 4198-5611 5516-8091. ______________________________________

The invention also relates to the use of these fragments as probes for the detection of HPV in tissue cultures.

Claims

1. An isolated DNA consisting of the 78 base sequence identified as 78-1 in FIG. 3.

8. A recombinant DNA consisting of a cloning vector and the isolated DNA of any of claims 1-5.

9. An isolated DNA of any one of claims 1-7 wherein said DNA is labelled with a label selected from the group consisting of a radioactive marker and a non-radioactive marker.

2. An isolated DNA consisting of the 156 base sequence identified as 78-1 and 78-2 in FIG. 3.

3. An isolated DNA consisting of a sequence extending from numbered nucleotide 3326 to numbered nucleotide 3575 in FIG. 1A, wherein said sequence consists of an E4 open reading frame.

4. An isolated DNA consisting of a sequence extending from numbered nucleotide 3842 to numbered nucleotide 4079 in FIG. 1B, wherein said sequence consists of an E5 open reading frame.

5. An isolated DNA encoding E6* protein consisting of a sequence extending from numbered nucleotide 229 to numbered nucleotide 404 in FIG. 1A.

6. An isolated DNA consisting of a sequence extending from numbered nucleotide 1275 to numbered nucleotide 1307 in FIG. 1A.

7. A recombinant DNA consisting of a cloning vector and the isolated DNA of claim 6.