Characterizing macromolecules interacting with at least three ligands on a sensor
First Claim
1. A method of discriminating between and mapping epitopes of a macromolecule by studying its interactions with ligands, comprising the steps of:
- determining blocking or non-blocking of the epitopes by the ligands by, after the macromolecule has interacted with at least one ligand, sequentially contacting the macromolecule with at least two additional ligands, either the macromolecule or one of the at least two additional ligands having been bound to a sensor surface;
determining ligand binding interaction by detecting a change in the physico-chemical properties of the sensor surface; and
discriminating between epitopes of the macromolecule and mapping their relative positions on the basis of the determined blocking or non-blocking of the epitopes by the ligands.
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Abstract
A method of characterizing a macromolecule by studying its interactions with ligands comprises the determination of the mutual influence of ligand interactions by, after the macromolecule has interacted with at least one ligand, contacting the macromolecule with at least one additional ligand, either the macromolecule or the additional ligand or ligands having been bound to a sensor surface, determining interaction by detecting a consequential change in the physico-chemical properties of the sensor surface, and on the basis of the determined mutual dependence between the ligand interactions discriminating between epitopes of the macromolecule and mapping their relative positions.
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Citations
14 Claims
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1. A method of discriminating between and mapping epitopes of a macromolecule by studying its interactions with ligands, comprising the steps of:
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determining blocking or non-blocking of the epitopes by the ligands by, after the macromolecule has interacted with at least one ligand, sequentially contacting the macromolecule with at least two additional ligands, either the macromolecule or one of the at least two additional ligands having been bound to a sensor surface; determining ligand binding interaction by detecting a change in the physico-chemical properties of the sensor surface; and discriminating between epitopes of the macromolecule and mapping their relative positions on the basis of the determined blocking or non-blocking of the epitopes by the ligands. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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Specification