Method for assaying urease

• US 5,556,760 A
• Filed: 01/31/1995
• Issued: 09/17/1996
• Est. Priority Date: 06/02/1994
• Status: Expired due to Fees

• Current Assignee: Biosensor Research Laboratories Co., Ltd.
• Original Assignee: Biosensor Research Laboratories Co., Ltd.
• Inventors: Kato, Takuji, Ito, Shigeji, Koori, Yoshihiro, Nakamura, Michihiro, Oka, Keiko, Tsuruta, Hitoshi, Matsui, Satomi
• Primary Examiner(s): Gitomer, Ralph J.
• Application Number:
  US08/381,000
• Time in Patent Office: 595 Days
• Field of Search: 435/12, 435/4, 128/642, 128/662.03, 128/662.06, 204/153.21, 204/403, 424/94.6, 604/280
• US Class Current: 435/12
• CPC Class Codes: A61B 5/14539 for measuring pH
• CPC Class Codes: C12Q 1/00 Measuring or testing processes involving enzymes, nucleic acids or microorganisms measuring or testing apparatus with condition measuring or sensing means, e.g. colony counters, C12M1/34; Compositions therefor; Processes of preparing such compositions
• CPC Class Codes: C12Q 1/58 involving urea or urease

Abstract

A method for assaying an enzyme on a targeted solid body surface, including the steps of contacting an end opening of a thin tube with a targeted solid body surface to form a space between the targeted solid body surface and inner wall surface of the thin tube, supplying a substrate solution into the space to bring the substrate solution in contact with the targeted solid body surface, and determining a pH change of the substrate solution in the space by using a pH electrode; and an apparatus therefor.

Claims

1. A method for assaying urease on a targeted solid body surface comprising:

(a) contacting an end opening of a thin tube with a targeted solid body surface to form a space between said targeted solid body surface and an inner wall surface of said thin tube;

(b) supplying a urea solution into said space to bring said urea solution into contact with said targeted solid body surface;

(c) determining a pH change of said urea solution in said space with a pH electrode, said pH electrode comprising a sensing portion whose tip is arranged in said space to be not more than 5 mm away from said end opening of said thin tube; and

(d) correlating the pH change determined in step (c) with the presence of urease where an increase in pH indicates the presence of urease.

2. The method according to claim 1, wherein the targeted solid body surface is a surface of a solid body selected from the group consisting of stomach, duodenum, small intestine, large intestine, rectum, urethra, oviduct, bronchium, gingiva, and a solid culture medium.

3. The method according to claim 1, wherein the targeted solid body surface is an inner wall surface of the stomach or the duodenum.

4. The method according to claim 1, wherein the distance between said tip of said sensing portion and said end opening of said thin tube is 0.1 to 1.0 mm.

5. The method according to claim 1, wherein the pH electrode is a pH-sensitive field effect transistor.

6. The method according to claim 1, wherein the thin tube is inserted into an endoscope.

7. The method of claim 1, wherein said space can be filled with said substrate solution.