Device for the simultaneous determination of several analytes

• US 5,556,789 A
• Filed: 07/01/1994
• Issued: 09/17/1996
• Est. Priority Date: 07/15/1993
• Status: Expired due to Fees

• Current Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Original Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Inventors: Baer, Reinhard, Lerch, Rolf, Goerlach-Graw, Ada
• Primary Examiner(s): Housel, James C.
• Assistant Examiner(s): FREED, RACHEL
• Application Number:
  US08/270,162
• Time in Patent Office: 809 Days
• Field of Search: 436/165, 436/169, 436/901, 436/63, 436/807, 422/56-58, 422/61, 435/7.1, 435/810
• US Class Current: 436/169
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/084 Passive control of flow resistance
• CPC Class Codes: B01L 3/5023 with a sample being transported to, and subsequently stored in an absorbent for analysis
• CPC Class Codes: Y10S 436/807 Apparatus included in process claim, e.g. physical support structures
• CPC Class Codes: Y10S 436/901 Drugs of abuse, e.g. narcotics, amphetamine

Abstract

Device for the determination of analytes having a sample application point, several separate sample withdrawal zones that are each connected with the sample application point by one capillary transport path and that have several test elements for the individual determination of analytes wherein a retardation zone is provided on at least one of the transport paths. Material for the capillary transport of a liquid sample defines a sample application zone and a plurality of sample withdrawal zones connected to said application zone by a like plurality of transport paths. At least one of said paths has a retardation zone which assures that liquid applied to said application zone arrives simultaneously at said withdrawal zones, regardless of the lengths of the paths.

Claims

1. Device for delivery of a liquid sample to multiple withdrawal zones at substantially the same time, said device comprising

a plurality of sample withdrawal zones,

fibrous capillary active transport means comprising a sample application zone and a plurality of transport paths connecting respective said sample withdrawal zones to said sample application zone, said fibrous capillary active transport means being free of reagents which react with analytes to be determined, and

retardation means provided in at least one of said transport paths and arranged so that liquid sample applied to said sample application zone arrives simultaneously at each of said sample withdrawal zones.

2. Device as in claim 1 wherein said transport paths all have the same length.

3. Device as in claim 1 wherein said transport paths extend radially from said application zone.

4. Device as in claim 1 wherein said transport paths do not have the same length.

5. Device as in claim 4 wherein at least one of said transport paths does not have retardation means therein, said retardation means in the at least one of the other transport paths comprising a smaller cross section than the cross section of said at least one transport path without retardation means.

6. Device as in claim 4 wherein said retardation means comprises fibrous capillary active material that retards liquid transport as compared to the rest of the transport path in which said retardation means is provided.

7. Device as in claim 1 wherein said transport paths do not all have the same length, said paths including a shortest path having retardation means and at least one longer path having no retardation means.

8. Device as in claim 1 wherein said fibrous capillary active transport means is a fleece.

9. Device as in claim 1 wherein said fibrous capillary active transport means is a fabric.

10. Method for the determination of several analytes contained in a liquid sample, said method comprising the steps of

providing fibrous capillary active transport means comprising a sample application zone, plurality of sample withdrawal zones spaced from said sample application zone, a plurality of transport paths connecting respective said sample withdrawal zones to said sample application zone, and retardation means provided in at least one of said transport paths and arranged so that liquid sample applied to said sample application zone arrives simultaneously at each of said sample withdrawal zones, and wherein said fibrous capillary active transport means is free of reagents which react with analytes to be determined,

applying liquid sample comprising analytes to said application zone,

contacting test elements containing reagents to at least two of said sample withdrawl zones, said reagents reacting with said analytes to produce observable results, and

determining said analytes in accordance with said results.