Monoclonal antibody recognizing a surface molecule on a subset of antigen-stimulated T cells and on certain malignancies of T and B cell origin

• US 5,556,947 A
• Filed: 08/09/1994
• Issued: 09/17/1996
• Est. Priority Date: 08/21/1992
• Status: Expired due to Term

• Current Assignee: United States Department Of Health And Human Services
• Original Assignee: United States Department Of Health And Human Services
• Inventors: Nelson, David L., Fleisher, Thomas A., Bock, Glenn H., Kurman, Carole C.
• Primary Examiner(s): Parr, Margaret
• Assistant Examiner(s): Gambel, Phillip
• Application Number:
  US08/287,718
• Time in Patent Office: 770 Days
• Field of Search: 530/387.1, 530/387.7, 530/388.1, 530/388.2, 530/388.7, 530/388.73, 530/388.75, 530/388.8, 530/388.85, 530/391.1, 530/391.3, 530/391.7, 435/70.21, 435/172.2, 435/240.27
• US Class Current: 530/391.3
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 16/3061 Blood cells
• CPC Class Codes: C07K 2317/73 Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
• CPC Class Codes: C12N 5/0694 Cells of blood, e.g. leukemia cells, myeloma cells

Abstract

This invention provides an IL-6 dependent B-cell lymphoma cell line designated DS-1 deposited with the American Type Culture Collection, wherein the cell line is reactive with a monoclonal antibody produced by a hybridoma cell line designated 10D2F6 deposited with the American Type Culture Collection. The invention also provides a purified antigen reactive with a monoclonal antibody produced by 10D2F6. The antigen also exists on DS-1. Also provided is a method of detecting the presence of an antigen-stimulated T-cell comprising detecting the presence of the antigen on a lymphocyte. In addition, the invention provides a method of detecting the presence of a neoplastic cell comprising detecting the presence of the antigen on a cell which is not normal a T-cell.

Claims

1. A purified antibody which specifically binds the same antigen as the monoclonal antibody produced by the hybridoma cell line designated 10D2F6 having ATCC Accession No. HB 11103, wherein the purified antibody binds an antigen which exists on an IL-6 dependent B-cell lymphoma cell line designated DS-1 having ATCC Accession No. CRL 11102 wherein the purified antibody also specifically binds an antigen on antigen-stimulated T cells, but not on non-stimulated T cells.

2. The antibody of claim 1, labeled with a detectable moiety.

3. The antibody of claim 1, bound to a solid support.

4. The antibody of claim 1, conjugated to a cytotoxic moiety.