Method and apparatus for post-transfer assaying of material on solid support
First Claim
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1. A device for treating reactant-carrying bodies with predetermined sequences of reagents, comprising:
- a housing;
a reagent pouch container which is receivable in said housing and is manually removable therefrom without tools;
at least one reagent pouch which is receivable in said reagent pouch container and is manually removable therefrom without tools, is capable of independently containing a quantity of a fluid reagent, and is provided with a valved conduit coupling capable of passing fluid only when coupled to another valved conduit coupling;
at least one flexible tubular conduit which is provided at each end with one of said valved conduit couplings;
a reactant-carrying body container which is receivable in said housing and is manually removable therefrom without tools;
at least one electrically operable pinch valve which is contained in said housing;
at least one transfer conduit for conducting fluid reagent from one of said reagent pouches to said reactant-carrying body container, which transfer conduit includes one of said flexible tubular conduits which passes through one of said pinch valves;
pressurized gas source means for supplying gas under superambient pressure to the interior of said reagent pouch container, whereby to compress all of the reagent pouches which are contained within said reagent pouch container; and
programmable control means for controlling the operation of said pinch valves.
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Abstract
A method and an apparatus useful as a secondary level assaying tool for qualitative and quantitative confirmation of results obtained by primary separation techniques and their correlation with a specific function or property of the tested sample. An apparatus is useful for the post-transfer development assaying on the solid support of the biological samples already assayed by standard separation techniques such as blotting or chromatography.
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Citations
9 Claims
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1. A device for treating reactant-carrying bodies with predetermined sequences of reagents, comprising:
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a housing; a reagent pouch container which is receivable in said housing and is manually removable therefrom without tools;
at least one reagent pouch which is receivable in said reagent pouch container and is manually removable therefrom without tools, is capable of independently containing a quantity of a fluid reagent, and is provided with a valved conduit coupling capable of passing fluid only when coupled to another valved conduit coupling;at least one flexible tubular conduit which is provided at each end with one of said valved conduit couplings; a reactant-carrying body container which is receivable in said housing and is manually removable therefrom without tools; at least one electrically operable pinch valve which is contained in said housing; at least one transfer conduit for conducting fluid reagent from one of said reagent pouches to said reactant-carrying body container, which transfer conduit includes one of said flexible tubular conduits which passes through one of said pinch valves; pressurized gas source means for supplying gas under superambient pressure to the interior of said reagent pouch container, whereby to compress all of the reagent pouches which are contained within said reagent pouch container; and programmable control means for controlling the operation of said pinch valves. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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Specification