Method of using liquid column packing materials

• US 5,559,039 A
• Filed: 08/18/1994
• Issued: 09/24/1996
• Est. Priority Date: 12/30/1992
• Status: Expired due to Term

• Current Assignee: Dow, Inc.
• Original Assignee: Dow, Inc.
• Inventors: Williams, Dwight E.
• Primary Examiner(s): Snay, Jeffrey
• Assistant Examiner(s): Ludlow, Jan M.
• Application Number:
  US08/292,520
• Time in Patent Office: 768 Days
• Field of Search: 210/198.2, 210/656, 502/403, 530/413, 530/811, 530/812, 530/816, 435/176, 435/177, 435/181, 436/518, 436/524, 436/527, 436/528, 436/532, 436/161
• US Class Current: 436/161
• CPC Class Codes: B01J 20/28078 Pore diameter
• CPC Class Codes: B01J 20/287 Non-polar phases; Reversed phases
• CPC Class Codes: B01J 20/289 bonded via a spacer
• CPC Class Codes: B01J 20/3204 Inorganic carriers, supports or substrates
• CPC Class Codes: B01J 20/3219 involving a particular spacer or linking group, e.g. for attaching an active group
• CPC Class Codes: B01J 20/3274 Proteins, nucleic acids, polysaccharides, antibodies or antigens
• CPC Class Codes: B01J 20/3282 Crosslinked polymers
• CPC Class Codes: B01J 2220/54 Sorbents specially adapted for analytical or investigative chromatography
• CPC Class Codes: B01J 2220/58 Use in a single column
• CPC Class Codes: B01J 31/0254 on mineral substrates
• CPC Class Codes: B01J 31/08 Ion-exchange resins
• CPC Class Codes: Y10S 530/811 Peptides or proteins is immobilized on, or in, an inorganic carrier
• CPC Class Codes: Y10S 530/812 Peptides or proteins is immobilized on, or in, an organic carrier

Abstract

Packing materials for liquid chromatographic or catalytic columns are prepared by contacting a porous protein-adsorptive particulate or membranous support, such as a porous silica particulate support, with an aqueous solution into which a protein has been dissolved to form a saturated coating of protein on the external surfaces of the porous protein-adsorptive support, removing excess protein that remains in solution by washing, and, then crosslinking the protein in the coating. The result is a packing material which resists further adsorption by many different proteins but which continues to provide the adsorptive or catalytic properties of the groups on the internal surfaces of the porous protein-adsorptive support for separations, analysis, or alteration of small molecules. The packing material of the present invention is particularly useful in HPLC or solid phase extraction columns for direct injection drug analysis in plasma, serum, and urine.

Claims

1. A method of analysis by liquid chromatography, comprising:

a) preparing an analyte containing biological fluid to be analyzed;

b) contacting said analyte with a liquid chromatography column packed with a packing material comprising a porous protein-adsorptive support having a coating of crosslinked protein on the external surfaces thereof, said coating of crosslinked protein being a relatively thin but saturated one, such that the signal obtained by at least one surface selective analytical method for nitrogen due to the saturated crosslinked protein coating does not exceed 67 percent of that for a bulk amount of said crosslinked protein; and

,

c) measuring said analyte as said analyte is passed through said chromatography column.

2. The method of claim 1 wherein said porous protein-adsorptive support is a porous silica support.

3. The method of claim 2 where the crosslinked protein coating consists of between 2.0/D and 10.0/D weight percent of the material, where D is the support particle diameter in micrometers.

4. The method of claim 3 wherein said protein is selected from the group consisting of serum albumin, bovine serum albumin, egg conalbumin, ovalbumin, and serum α

-glycoprotein, and wherein said protein is crosslinked with a cross-linking agent selected from the group consisting of glutaraldehyde, N, N'"'"' dimethylaminopropyl ethyl-carbodiimide, formaldehyde, and bis (sulfosuccinimidyl) suberate.