DNA and DNA technology for the construction of networks to be used in chip construction and chip production (DNA-chips)

• US 5,561,071 A
• Filed: 09/25/1995
• Issued: 10/01/1996
• Est. Priority Date: 07/24/1989
• Status: Expired due to Term

• Current Assignee: Cornelis P. Hollenberg, Ernesto Di Mauro
• Original Assignee: Cornelis P. Hollenberg, Ernesto Di Mauro
• Inventors: Hollenberg, Cornelis P., di Mauro, Ernesto
• Primary Examiner(s): Jones, W. Gary
• Assistant Examiner(s): Marschel, Ardin H.
• Application Number:
  US08/532,542
• Time in Patent Office: 372 Days
• Field of Search: 435/6, 435/810, 435/287, 435/299, 536/22.1, 536/25.3, 437/1, 437/15, 437/16, 437/35, 437/36, 437/38, 437/51, 437/54, 437/80, 437/225, 437/238, 935/77, 935/88
• US Class Current: 438/503
• CPC Class Codes: B82Y 10/00 Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
• CPC Class Codes: G06N 3/123 DNA computing
• CPC Class Codes: H01L 21/32 using masks
• CPC Class Codes: H01L 25/50 Multistep manufacturing processes of assemblies consisting of devices, each device being of a type provided for in group H01L27/00 or H01L29/00 H01L21/50 takes precedence
• CPC Class Codes: H01L 2924/00 Indexing scheme for arrangements or methods for connecting or disconnecting semiconductor or solid-state bodies as covered by H01L24/00
• CPC Class Codes: H01L 2924/0002 Not covered by any one of groups H01L24/00, H01L24/00 and H01L2224/00
• CPC Class Codes: H10K 10/701 Organic molecular electronic devices
• CPC Class Codes: H10K 85/761 Biomolecules or bio-macromolecules, e.g. proteins, chlorophyl, lipids or enzymes
• CPC Class Codes: Y10S 435/81 Packaged device or kit
• CPC Class Codes: Y10S 438/944 Shadow
• CPC Class Codes: Y10S 438/945 Special, e.g. metal

Abstract

The invention relates to construction of specific molecular microcircuits by the use of double and single stranded nucleic acids and specific DNA-binding proteins.

Claims

1. A method for the production of a nucleic acid chip which method comprises:

(a) providing a substrate;

(b) depositing onto said substrate two or more sequence-specific DNA-binding proteins in a predetermined deposition pattern having beginning and ending points; and

(c) attaching to the predeposited proteins on the substrate by site-specific deposition and orientation a preconstructed nucleic acid sequence such that the beginning of said sequence attaches to said beginning point protein and the ending of said sequence attaches to said ending point protein to construct a network in said chip.

2. A method according to claim 1 wherein the nucleic acid chip produced is an electronic microcircuit.

3. A method according to claim 1 wherein the network further comprises nucleic acid formed by DNA and RNA synthesis reactions and hybridization to the preconstructed nucleic acid sequence.

4. A method according to claim 1 which further comprises depositing electrically conductive substances onto the network.

5. A method according to claim 1 further comprising

(a) shadowing under low angle with a masking substance so that the substrate stays free of shadow under and one side of the nucleic acid sequences of the network;

and

(b) depositing a metallo-organic chemical vapour using electron conductive material such as doped gallium arsenide and doped silicium on the track which remains free from the masking substance under and/or beside said nucleic acid sequences of the network.

6. A method for the construction of a mask for the production of a nucleic acid chip by a photolithographic procedure, comprising the steps:

(a) producing a nucleic acid chip according to claim 1; and

(b) shadowing under low angle with a masking, electron dense substance so that the substrate stays free of shadow under and beside the nucleic acid sequences of the network.

7. A method for the construction of a mask for the production of a nucleic acid chip by a photolithographic procedure, comprising the steps:

(a) producing a nucleic acid chip according to claim 1; and

(b) converting the network in said chip into an electron dense substance.