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Method for refolding misfolded enzymes with detergent and cyclodextrin

  • US 5,563,057 A
  • Filed: 10/31/1994
  • Issued: 10/08/1996
  • Est. Priority Date: 10/31/1994
  • Status: Expired due to Term
First Claim
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1. A method for folding an enzyme from a first misfolded configuration where the enzyme is completely defective in enzymatic activity to a second, native and active configuration where the enzyme has enzymatic activity, and then at least partially purifying the enzyme, the method comprising:

  • adding a detergent having a linear alkyl non-polar portion to the enzyme to form a detergent-enzyme complex, where immediately prior to the addition of the detergent, the enzyme is in said first configuration;

    contacting the detergent-enzyme complex with a cyclodextrin in an aqueous solution whereby the enzyme assumes the second active configuration; and

    then at least partially purifying the enzyme that is in the second active configuration by separating it from the detergent.

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