Cell potential measurement apparatus having a plurality of microelectrodes
First Claim
1. A cell potential measurement apparatus for measurement of electrical physiological characteristics of cells, comprising:
- (A) an integrated cell holding instrument provided with a plurality of microelectrodes arranged in a matrix form on the surface of a glass plate, conductive patterns connected to the microelectrodes, electric contact points which are connected to edge parts of these conductive patterns, an insulation coating covering the surface of said conductive patterns, said microelectrodes being in electrical connection to a cell holding part which is constructed so as to contain cells and arranged in an area including said plurality of microelectrodes, and an electric connection means for providing an electric signal to said microelectrodes and for leading out an electric signal from said microelectrodes, said electric connection means including a half-split holder which has contacts touching said electric contact points and fixes said glass plate by holding the plate at the top and bottom of the plate;
(B) a stimulation signal supply means to be connected the electric connection means of said integrated cell holding instrument for providing electric stimulation to said cells; and
(C) a signal processing means to be connected to the electric connection means of said integrated cell holding instrument for processing an output signal arising from electric physiological activities of said cells.
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Accused Products
Abstract
A cell potential measurement apparatus, which uses a planar electrode enabling a multi-point simultaneous measurement of potential change arising from cell activities, is provided which can conduct measurements accurately and efficiently as well as can improve convenience of arranging measurement results. According to the configuration of the cell potential measurement apparatus of this invention, it includes an integrated cell holding instrument, which includes a planar electrode provided with a plurality of microelectrodes arranged in a matrix form on the surface of a substrate, a cell holding part for placing cells thereon, drawer patterns from the microelectrodes, and electric contact points for outside connections; an optical observation means for optical observations of cells; a stimulation signal supply means to be connected to the cell holding instrument for providing electric stimulation to the cells; and a signal processing means to be connected to the cell holding instrument for processing an output signal arising from electric physiological activities of the cells. It is preferable that a cell culturing means is also provided for maintaining a culture atmosphere of the cells placed on the integrated cell holding instrument.
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Citations
13 Claims
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1. A cell potential measurement apparatus for measurement of electrical physiological characteristics of cells, comprising:
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(A) an integrated cell holding instrument provided with a plurality of microelectrodes arranged in a matrix form on the surface of a glass plate, conductive patterns connected to the microelectrodes, electric contact points which are connected to edge parts of these conductive patterns, an insulation coating covering the surface of said conductive patterns, said microelectrodes being in electrical connection to a cell holding part which is constructed so as to contain cells and arranged in an area including said plurality of microelectrodes, and an electric connection means for providing an electric signal to said microelectrodes and for leading out an electric signal from said microelectrodes, said electric connection means including a half-split holder which has contacts touching said electric contact points and fixes said glass plate by holding the plate at the top and bottom of the plate; (B) a stimulation signal supply means to be connected the electric connection means of said integrated cell holding instrument for providing electric stimulation to said cells; and (C) a signal processing means to be connected to the electric connection means of said integrated cell holding instrument for processing an output signal arising from electric physiological activities of said cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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Specification