Method, reagent and kit for the detection of fecal occult blood
First Claim
1. A method for detecting occult blood in a specimen comprising combining the specimen with(a) an oxidizable substrate that produces a colored product in the presence of peroxide and hemoglobin;
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Abstract
A fecal specimen may be tested for the presence of occult blood by combining the fecal specimen with
(a) an oxidizable substrate that produces a colored product in the presence of peroxide and hemoglobin;
(b) hydrogen peroxide or a peroxide source; and
(c) an enhancer selected to enhance the sensitivity of the test. Suitable enhancers are monocyclic nitrogen-containing aromatic heterocyclic compounds; tertiary or quaternary ammonium compounds having a phenyl, hydroxy alkyl or esterified hydroxy alkyl attached to the nitrogen; or quinoline or a substituted derivative thereof. If hemoglobin is present in the fecal sample, the oxidizable substrate is converted to the visibly detectable, colored product.
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Citations
17 Claims
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1. A method for detecting occult blood in a specimen comprising combining the specimen with
(a) an oxidizable substrate that produces a colored product in the presence of peroxide and hemoglobin; - and
(b) a liquid developer comprising hydrogen peroxide or a peroxide source, a carrier consisting essentially of water and ethanol, and an enhancer component, said enhancer component consisting of a material selected from the group consisting of tertiary and quaternary amines having a hydroxy alkyl or esterified hydroxy alkyl group attached to the nitrogen, wherein the oxidizable substrate and liquid developer are combined with the specimen in amounts effective to produce a visually detectable color change if medically significant amounts of blood are present in the specimen. - View Dependent Claims (2, 3, 4, 5)
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6. A composition for use as a developer in a test for occult blood based upon the oxidation of a substrate to a colored product comprising
(a) hydrogen peroxide or a peroxide source; -
(b) an amount of an enhancer component effective to enhance the amount of colored product produced; and (c) a carrier consisting essentially of water and ethanol, wherein the enhancer component consists of a material selected from the group consisting of tertiary and quaternary amines having a hydroxy alkyl or esterified hydroxy alkyl group attached to the nitrogen. - View Dependent Claims (7, 8, 9, 10, 11)
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12. A kit for the detection of occult blood comprising, in packaged combination,
(a) an oxidizable substrate which is converted to a colored product in the presence of peroxide and hemoglobin; - and
(b) a liquid developer comprising hydrogen peroxide or a peroxide source and an enhancer component in an amount effective to enhance the conversion of the oxidizable substrate to the colored product in a carrier consisting essentially of ethanol and water, wherein the enhancer component consists of a material selected from the group consisting of tertiary and quaternary amines having a hydroxy alkyl or esterified hydroxy alkyl group attached to the nitrogen. - View Dependent Claims (13, 14, 15, 16, 17)
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Specification