Method of photometric in vitro determination of the content of oxygen in a blood sample
First Claim
1. A method for the photometric in vitro determination of the content of oxygen in a blood sample, comprising:
- (a) transferring the blood sample to a sample container;
(i) having an inlet opening and otherwise being essentially sealed; and
(ii) incorporating a luminophor in a polymer matrix, whose luminescence is quenched in the presence of oxygen;
(b) disposing the sample container in optical communication with an optical system comprising a radiation source and a radiation detector for detecting radiation transmitted from said source;
(c) transmitting radiation from the radiation source to the sample container to excite the luminophor;
(d) transmitting the luminescence emitted by the excited luminophor to the radiation detector; and
(e) detecting and registering the luminescence.
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Abstract
In a method of photometric in vitro determination of the content of oxygen in a blood sample, a blood sample is transferred directly from an in vivo locality to an at least partially transparent sample container of a sampling device. The sample container has a measuring chamber containing a luminophor, the luminescence of which is quenched in the presence of oxygen. The luminophor is excited by irradiation with radiation from a radiation source. The luminescence emitted by the excited luminophor is detected by a radiation detector and the oxygen content determined on the basis of the radiation detected by the radiation detector. A sampling device and a system for photometric in vitro determination of oxygen in a blood sample are also described.
37 Citations
17 Claims
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1. A method for the photometric in vitro determination of the content of oxygen in a blood sample, comprising:
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(a) transferring the blood sample to a sample container; (i) having an inlet opening and otherwise being essentially sealed; and (ii) incorporating a luminophor in a polymer matrix, whose luminescence is quenched in the presence of oxygen; (b) disposing the sample container in optical communication with an optical system comprising a radiation source and a radiation detector for detecting radiation transmitted from said source; (c) transmitting radiation from the radiation source to the sample container to excite the luminophor; (d) transmitting the luminescence emitted by the excited luminophor to the radiation detector; and (e) detecting and registering the luminescence.
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2. A sample container for the photometric in vitro determination of the oxygen content in a blood sample, said sample container:
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(a) having an inlet opening and otherwise being essentially sealed; and (b) incorporating a luminophor in a polymer matrix whose luminescence is quenched in the presence of oxygen. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9)
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10. A system for the photometric in vitro determination of the content of oxygen in a blood sample, comprising:
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(a) a sample container; (i) having an inlet opening and otherwise being essentially sealed; and (ii) incorporating a luminophor in a polymer matrix, whose luminescence is quenched in the presence of oxygen; and (b) an analyzer; (i) having an optical system incorporating a radiation source and a radiation detector for detecting radiation transmitted from the radiation source; (ii) incorporating means for providing optical communication between the optical system and the sample container so that radiation transmitted from the radiation source is transmitted to the sample container such that the luminophor is excited and the luminescence emitted by the excited luminophor is transmitted to and detected by the radiation detector; and (iii) incorporating means for measuring the luminescence detected by the radiation detector. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17)
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Specification