Method of detecting biological materials using a polyvinyllidene fluoride membrane

• US 5,567,626 A
• Filed: 04/11/1995
• Issued: 10/22/1996
• Est. Priority Date: 04/11/1995
• Status: Expired due to Term

• Current Assignee: Pall Corporation (Danaher Corporation)
• Original Assignee: Pall Corporation (Danaher Corporation)
• Inventors: Salinaro, Richard F.
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): CARRILLO, BIBI SHARIDAN
• Application Number:
  US08/420,218
• Time in Patent Office: 560 Days
• Field of Search: 436/86, 436/164, 436/166, 436/168, 436/169, 436/178, 436/531, 435/6
• US Class Current: 436/178
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: G01N 33/5436 with ligand physically entrapped within the solid phase liposomes G01N33/5432; immunological test elements G01N33/54386
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

The present invention provides an improved method of detecting or transferring a biological material whereby a biological material is adhered to a membrane which is then contacted with a detecting reagent, wherein the improvement comprises utilizing a polyvinylidene fluoride membrane which has been subjected to a temperature of at least about 80° C. but less than the temperature at which the membrane softens and deforms for a time sufficient to reduce the ability of the detecting reagent to directly adhere to the membrane.

Claims

1. A method of detecting a biological material whereby a biological material is adhered to a membrane which is then contacted with a reagent capable of binding to said biological material and capable of being detected, such that the detection of said reagent bound to-said biological material represents a biological material detection signal evidencing the presence of said biological material, and the detection of said reagent directly bound to said membrane represents background noise, wherein the improvement comprises utilizing a polyvinylidene fluoride membrane which has been subjected to a temperature of at least about 80°

C. but less than the temperature at which the membrane softens and deforms for a time sufficient to substantially reduce said detecting reagent from directly adhering to said membrane, so as to result in a decrease in the background noise and an increase in a biological material detection signal-to-noise ratio.

2. The method of claim 1, wherein said membrane is subjected to a temperature of about 80°

C. to about 160°

C.

3. The method of claim 2, wherein said membrane is subjected to said temperature for about 5 minutes to about 64 hours.

4. The method of claim 1, wherein said membrane is subjected to a temperature of about 80°

C. to about 150°

C.

5. The method of claim 1, wherein said membrane is subjected to a temperature of about 100°

C. to about 150°

C.

6. The method of claim 5, wherein said membrane is subjected to said temperature for about 32 hours or more.

7. The method of claim 1, wherein said membrane is subjected to a temperature of about 120°

C. to about 150°

C.

8. The method of claim 7, wherein said membrane is subjected to said temperature for about 16 hours or more.

9. The method of claim 1, wherein said membrane is subjected to a temperature of about 135°

C. to about 145°

C.

10. The method of claim 9, wherein said membrane is subjected to said temperature for about 12 hours or more.

11. The method of claim 10, wherein said membrane is subjected to said temperature for about 24 hours or more.

12. The method of claim 1, wherein said membrane is subjected to said temperature by contacting said membrane with a heated surface.

13. The method of claim 1, wherein said membrane is subjected to said temperature by placing said membrane in a circulating air oven.

14. The method of claim 13, wherein said membrane is in the form of a roll when placed in said circulating air oven.

15. The method of claim 1, wherein said membrane is porous and has a pore rating of about 1 μ

m or less.

16. The method of claim 15, wherein said membrane has a pore rating of about 0.5 μ

m or less.

17. The method of claim 16, wherein said membrane has a pore rating of about 0.05-0.45 μ

m.

18. The method of claim 17, wherein said membrane has a pore rating of about 0.05-0.2 μ

m.