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Separation media for electrophoresis

  • US 5,569,364 A
  • Filed: 05/10/1994
  • Issued: 10/29/1996
  • Est. Priority Date: 11/05/1992
  • Status: Expired due to Term
First Claim
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1. In an electrophoretic device comprising an electrophoretic medium, the improvement which comprises:

  • an electrophoretic medium comprising a dispersion of particulate chemically-crosslinked microgels having particle sizes with a distribution of particles no smaller than about 10 nm and no larger than about 2 mm, wherein the weight concentration of said microgels in said dispersion is between about 1% and 50% and wherein said medium is reversibly convertible between a pumpable low viscosity state and an electrophoretically sieving high viscosity state, said high viscosity state characterized by a viscosity of at least 100% greater than the viscosity of said medium in said low viscosity state, and wherein the conversion of said low viscosity state to said high viscosity state can be affected by a change in temperature, pH, solvent concentration, ionic strength, presence and concentration of a specific ion, or other property or combinations of properties of said medium.

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