Apparatus for separating a mixture

• US 5,569,367 A
• Filed: 01/27/1995
• Issued: 10/29/1996
• Est. Priority Date: 04/16/1992
• Status: Expired due to Term

• Current Assignee: British Technology Group Ltd. (Boston Scientific Corporation)
• Original Assignee: British Technology Group Ltd. (Boston Scientific Corporation)
• Inventors: Betts, Walter B., Hawkes, Jeremy J.
• Primary Examiner(s): Gorgos, Kathryn
• Assistant Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US08/318,786
• Time in Patent Office: 641 Days
• Field of Search: 204/299 R, 204/180.1, 204/183.1, 204/180.5, 204/547, 204/643
• US Class Current: 204/547
• CPC Class Codes: B01D 57/02 by electrophoresis treatment of water, waste water, sewage or sludge by electrophoresis C02F1/469; electrophoretic production of compounds or non-metals C25B7/00; investigating or analysing materials by using electrophoresis G01N27/26
• CPC Class Codes: B03C 5/026 using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
• CPC Class Codes: C12M 47/04 Cell isolation or sorting purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04
• CPC Class Codes: G01N 27/44773 Multi-stage electrophoresis, e.g. two-dimensional electrophoresis

Abstract

Apparatus for separating a mixture of particles comprising a chamber with an inlet and an outlet, means for providing an eluant flow between said inlet and said outlet, within the chamber an electrode structure of at least two electrodes, means for applying an alternating voltage between the electrodes, said electrode structure being such that when energized in operation of the apparatus there is established in the eluant a spatially non-uniform alternating field, whereby particles caused to flow through the chamber in said eluant flow are carried past the electrode structure and are separated into fractions. There may be an ancillary electrode structure of at least two electrodes placed upstream of the main electrode structure with regard to the direction of the flow of fluid. At least one of the electrodes of the electrode structure may be comprised of one or more grid like structures. The electrode structure may be comprised of interleaved grid like structures aligned to obstruct straight through flow.

Claims

1. Apparatus for separating a mixture of particles by selective retardation of some of the particles comprising:

a chamber with an inlet and an outlet,

means for providing an eluant flow between said inlet and said outlet,

an electrode structure of at least two electrodes disposed within the chamber,

means for applying an alternating voltage between the electrodes, said electrode structure being such that when energized in operation of the apparatus there is established in the eluant a spatially non-uniform alternating field, where particles caused to flow through the chamber in said eluant flow are carried past the electrode structure and are separated into fractions,

wherein an auxiliary electrode structure is placed upstream of the main electrode structure, the auxiliary electrode structure being energized in use, such that substantially all the particles are collected into a relatively small volume.

2. Apparatus as claimed in claim 1, wherein said means for providing the eluant flow through the chamber includes a pump.

3. Apparatus as claimed in claim 1, wherein said means for providing the eluant flow through the chamber uses the force of gravity.

4. Apparatus as claimed in claim 1 in which at least one of the electrodes of the electrode structure is comprised of one or more structures in the form of a grade.

5. Apparatus as claimed in claim 1, wherein the electrode structure comprises interleaved, structures in the form of a grid, aligned to obstruct straight through flow.

6. Method for separating a mixture of particles using an apparatus including a chamber;

a main electrode structure of at least two electrodes disposed within the chamber;

means for applying an alternating voltage between the electrodes, and an ancillary electrode structure disposed upstream of the main electrode structure, the method including;

providing a spatially non-uniform alternating electric field in the chamber,

providing a flow of eluant through the chamber and the electric field,

eluting a limited sample of said mixture with said eluant,

passing the eluted sample through the electric field,

causing or permitting the different types of particles in the mixture to be retarded differentially; and

producing a flow of fractions of different types of particles separated by the flow of eluant,

wherein a uniform starting band of mixture is formed upstream of the said spatially non-uniform field by energizing the ancillary electrode structure for a length of time with a voltage sufficiently high enough to collect particles in a starting band, and then reducing the voltage, thus causing or permitting the starting band to be washed along the chamber for separation.

7. Method as claimed in claim 6 including altering the frequency of the non-uniform alternating electric field to alter the order in which the particles are separated.

8. Method as claimed in claim 7, including altering the frequency of the non-uniform alternating electric field to alter the degree of separation between each of the fractions with a greater concentration of one particular type of particle to some extent.

9. Method as claimed in claim 6 including switching the non-uniform alternating electric field on and off to sharpen the fractions with a greater concentration of one particular type of particle.

10. A method for forming a uniform starting band in a process of separating a mixture of particles using an apparatus including a chamber;

an electrode structure of at least two electrodes disposed within the chamber; and

means for applying an alternating voltage between the electrodes, the method including;

forming the uniform starting band at the head of the electrode structure by passing a flow of particles in suspension through the electrode structure,

energizing the electrode structure for a defined length of time with a voltage high enough to collect particles in an initial part of the electrode structure as a band and then reducing the voltage,

causing or permitting the band of collected particles to be washed along the chamber for separation action.

11. Method as claimed in claim 10, wherein the electrode structure includes an ancillary electrode structure which is re-energized once the band of previously collected particles has been washed-off the ancillary electrode structure.