DNA comprising plum pox virus and tomato spotted wilt virus cDNAS for disease resistance
First Claim
1. DNA comprising a combination of fragment A linked directly or indirectly to fragment B, wherein:
- fragment A consists of a double-stranded cDNA fragment derived from the RNA of the plum pox virus; and
fragment B consists of a double-stranded cDNA fragment derived from the S RNA of the N structural gene of the tomato spotted wilt virus;
said combination, when expressed in a plant, conferring on said plant an increased resistance, as compared to a natural plant of the same species, to tomato spotted wilt virus and another tospovirus.
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Abstract
The present invention relates to novel, recombinant deoxyribonucleic acids (DNA), to vectors and host organisms which contain them, and to transgenic plants which contain the recombinant DNA and possess an elevated resistance toward pernicious organisms and plant diseases, where the recombinant deoxyribonucleic acids (DNA) are characterized in that they consist of a combination of the following components, or components possessing a DNA which in each case has the same effect, or contain these constituents:
(a) a double-stranded cDNA fragment which is derived from the RNA of the plum pox virus (PPV) ("fragment A"), and
(b) a double-stranded cDNA fragment which is derived from the S RNA of the tomato spotted wilt virus (TSWV) ("fragment B"), it being possible for these combinations also to possess, in addition to further DNA fragments which may optionally be present, a promoter which is active in plant cells.
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Citations
55 Claims
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1. DNA comprising a combination of fragment A linked directly or indirectly to fragment B, wherein:
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fragment A consists of a double-stranded cDNA fragment derived from the RNA of the plum pox virus; and fragment B consists of a double-stranded cDNA fragment derived from the S RNA of the N structural gene of the tomato spotted wilt virus; said combination, when expressed in a plant, conferring on said plant an increased resistance, as compared to a natural plant of the same species, to tomato spotted wilt virus and another tospovirus. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 27, 28, 33, 38, 51)
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20. DNA comprising SEQ ID NO:
- 1 or a DNA that hybridizes to SEQ ID NO;
1 under stringent hybridization conditions, said DNA that hybridizes to SEQ ID NO;
1, when expressed in a plant, conferring on said plant an increased resistance, as compared to a natural plant of the same species, to tomato spotted wilt virus and another tospovirus. - View Dependent Claims (21, 29, 34, 39, 43, 45, 47, 49, 52, 53)
- 1 or a DNA that hybridizes to SEQ ID NO;
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22. DNA comprising SEQ ID NO:
- 5 or a DNA that hybridizes to SEQ ID NO;
5 under stringent hybridization conditions, said DNA that hybridizes to SEQ ID NO;
5, when expressed in a plant, conferring on said plant an increased resistance, as compared to a natural plant of the same species, to tomato spotted wilt virus and another tospovirus. - View Dependent Claims (23, 31, 36, 41, 44, 46, 48, 50, 54, 55)
- 5 or a DNA that hybridizes to SEQ ID NO;
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24. Isolated DNA selected from the group consisting of SEQ ID NOS:
- 1 and 5.
- View Dependent Claims (25, 26)
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30. A transformed plant cell, said plant cell containing in its genome DNA in addition to the DNA naturally constituting the genome of said plant cell, said plant cell expressing said additional DNA, said additional DNA consisting of SEQ ID No:
- 1, and said transformed plant cell, as a result of the expression of said additional DNA, exhibiting an increased resistance, as compared to a plant cell of the same plant species and cell type that is not so transformed, to tomato spotted wilt virus and another tospovirus, or propagation material of said transformed plant cell.
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32. A transformed plant cell, said plant cell containing in its genome DNA in addition to the DNA naturally constituting the genome of said plant cell, said plant cell expressing said additional DNA, said additional DNA consisting of SEQ ID No:
- 5, and said transformed plant cell, as a result of the expression of said additional DNA, exhibiting an increased resistance, as compared to a plant cell of the same plant species and cell type that is not so transformed, to tomato spotted wilt virus and another tospovirus, or propagation material of said transformed plant cell.
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35. A transformed whole plant, said whole plant containing in its genome DNA in addition to the DNA naturally constituting the genome of said whole plant, said whole plant expressing said additional DNA, said additional DNA consisting of SEQ ID No:
- 1, and said transformed whole plant, as a result of the expression of said additional DNA, exhibiting an increased resistance, as compared to a whole plant of the same plant species that is not so transformed, to tomato spotted wilt virus and another tospovirus, or propagation material of said transformed whole plant.
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37. A transformed whole plant, said whole plant containing in its genome DNA in addition to the DNA naturally constituting the genome of said whole plant, said whole plant expressing said additional DNA, said additional DNA consisting of SEQ ID No:
- 5, and said transformed whole plant, as a result of the expression of said additional DNA, exhibiting an increased resistance, as compared to a whole plant of the same plant species that is not so transformed, to tomato spotted wilt virus and another tospovirus, or propagation material of said transformed whole plant.
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40. A transformed plant part, said plant part containing in its genome DNA in addition to the DNA naturally constituting the genome of said plant part, said plant part expressing said additional DNA, said additional DNA consisting of SEQ ID No:
- 1, and said transformed plant part, as a result of the expression of said additional DNA, exhibiting an increased resistance, as compared to a plant part of the same plant species and part type that is not so transformed, to tomato spotted wilt virus and another tospovirus, or propagation material of said transformed plant part.
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42. A transformed plant part, said plant part containing in its genome DNA in addition to the DNA naturally constituting the genome of said plant part, said plant part expressing said additional DNA, said additional DNA consisting of SEQ ID No:
- 5, and said transformed plant part, as a result of the expression of said additional DNA, exhibiting an increased resistance, as compared to a plant part of the same plant species and part type that is not so transformed, to tomato spotted wilt virus and another tospovirus, or propagation material of said transformed plant part.
Specification