Multilayered bioelectronic sensors

• US 5,571,568 A
• Filed: 06/07/1995
• Issued: 11/05/1996
• Est. Priority Date: 06/15/1989
• Status: Expired due to Fees

• Current Assignee: Biocircuits Corporation
• Original Assignee: Biocircuits Corporation
• Inventors: Ribi, Hans O., Guion, Todd, Shafer, Paul T.
• Primary Examiner(s): LUSIGNAN, MICHAEL R
• Application Number:
  US08/474,114
• Time in Patent Office: 517 Days
• Field of Search: 427/58, 427/374.1, 427/379, 427/385.5, 427/487
• US Class Current: 427/487
• CPC Class Codes: B01J 2219/00734 Lipids
• CPC Class Codes: G01N 33/542 with steric inhibition or signal modification, e.g. fluorescent quenching
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: Y10S 436/806 Electrical property or magnetic property

Abstract

Bioelectronic sensors are provided employing a thin surfactant polymeric electrically conducting layer to which may be bound members of specific binding pairs. Binding of an analyte or a reagent to the specific binding pair member layer may change the electrical, optical, or structural properties of the layer for measurement of analyte. The change in the polymeric layer provides for a sensitive measurement.

Claims

1. A method of forming a crystalline electrically conducting surfactant layer comprising:

spreading over an aqueous subphase on a solid substrate a solution of surfactant comprising polymerizable surfactants capable of forming an electrically conducting surfactant polymer, wherein said aqueous subphase comprises at least one water miscible organic compound;

evaporating the solvent of said surfactant solution to produce films;

heating said substrate at a temperature and for a time sufficient to cause surfactant melting;

cooling said melted dry patches to a temperature of less than about 10°

C.; and

polymerizing said polymerizable surfactants to said electrically conducting surfactant polymer.

2. A method according to claim 1, wherein said subphase comprises at least one of dimethyl sulfoxide and glycerol.

3. A method according to claim 2, wherein said subphase comprises from about 1 to 5 volume % of dimethyl sulfoxide.

4. A method according to claim 2, wherein said heating is to at least about 100°

C. and below the boiling point of said subphase.

5. A method according to claim 1, wherein said polymerization is with radiation.

6. A method according to claim 1, wherein said polymerizable surfactant is an alkadiyne of at least 6 carbon atoms.

7. A method according to claim 1, wherein said melting is at at least about 120°

C. and said cooling occurs in less than about one minute.

8. A method according to claim 1, wherein said surfactant layer comprises non-polymerizable non-volatile hydrocarbons or surfactants.