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.phi.29 DNA polymerase

  • US 5,576,204 A
  • Filed: 02/11/1993
  • Issued: 11/19/1996
  • Est. Priority Date: 03/24/1989
  • Status: Expired due to Term
First Claim
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1. A Φ

  • 29-type DNA polymerase produced from a non-naturally occurring DNA fragment consisting of DNA encoding a modified Φ

    29-type DNA polymerase, wherein said polymerase comprises sufficient DNA polymerase activity for use in DNA sequencing, and an exonuclease activity which is less than 10% the activity of the corresponding naturally occurring Φ

    29-type DNA polymerase.

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