Method for thermal cycling nucleic acid assays

• US 5,576,218 A
• Filed: 04/14/1995
• Issued: 11/19/1996
• Est. Priority Date: 01/11/1994
• Status: Expired due to Fees

• Current Assignee: Abbott Laboratories
• Original Assignee: Abbott Laboratories
• Inventors: Zurek, Thomas F., Hanley, Kathleen A., Pepe, Curtis J.
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): WALLENHORST, MAUREEN
• Application Number:
  US08/422,688
• Time in Patent Office: 585 Days
• Field of Search: 436/52, 436/53, 436/50, 436/55, 436/157, 436/174, 422/81, 422/82, 422/82.12, 422/93, 935/85-88, 165/12, 165/26, 165/30, 236/13, 435/285.1, 435/286.1, 435/287.1, 435/809
• US Class Current: 436/174
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: G05D 23/138 for gases G05D23/1393 takes precedence
• CPC Class Codes: Y10T 436/115831 Condition or time responsive
• CPC Class Codes: Y10T 436/117497 with a continuously flowing sample or carrier stream
• CPC Class Codes: Y10T 436/118339 with formation of a segmented stream
• CPC Class Codes: Y10T 436/12 Condition responsive control
• CPC Class Codes: Y10T 436/25 including sample preparation

Abstract

The method for thermal cycling of nucleic acid assays includes a blended fluid stream produced from a plurality of constant velocity, constant volume, constant temperature fluid streams wherein to provide a variable temperature, constant velocity, constant volume fluid stream which is introduced into a sample chamber for heating and cooling samples contained therein. By diverting and altering the ratio of the constant temperature fluid streams relative to one another, the blended fluid stream is rapidly variable in temperature, providing for almost instantaneous temperature change within the environment defined by the sample chamber.

Claims

1. A method for thermal cycling a sample comprising the steps of:

a. directing the flow of a composite stream into the environment of a sample to achieve a first sample-target-temperature, wherein said composite stream is composed of a plurality of component fluid streams each from a distinct source and each maintained at a different constant temperature;

b. maintaining said first sample-target-temperature for a preselected period;

c. adjusting said composite stream to achieve a second sample-target-temperature wherein when said first sample-target-temperature is less than said second sample-target-temperature, a sample-ramp-speed of at least 1.0°

C./second is achieved, and wherein said adjusting is achieved by selectively blending in varying ratios said component fluid streams; and

d. maintaining said second sample-target-temperature for a preselected period.

2. The method of claim 1 wherein said composite stream of step (a) has a temperature substantially the same as said first sample-target-temperature.

3. The method of claim 1 wherein said composite stream of step (a) initially has a temperature disparate from said first sample-target-temperature and after said sample substantially achieves said first sample-target-temperature, said composite stream is altered to a temperature substantially the same as said first sample-target-temperature.

4. The method of claim 3 wherein said composite stream of step (a) has an initial temperature that is greater than said first sample-target-temperature.

5. The method of claim 3 wherein said composite stream of step (a) has an initial temperature that is less than said first sample-target-temperature.

6. The method of claim 1 wherein said adjusted composite stream of step (c) has a temperature substantially the same as said second sample-target-temperature.

7. The method of claim 1 wherein said adjusted composite stream of step (c) initially has a temperature disparate from said second sample-target-temperature and after said sample substantially achieves said second sample-target-temperature said adjusted composite stream is altered to a temperature substantially the same as said second sample-target-temperature.

8. The method of claim 7 wherein said adjusted composite stream of step (c) has an initial temperature that is greater than said second sample-target-temperature.

9. The method of claim 7 wherein said adjusted composite stream of step (c) has an initial temperature that is less than said second sample-target-temperature.

10. The method of claim 1 wherein said plurality of component fluid streams comprises at least one component fluid stream at a temperature higher than ambient temperature.

11. The method of claim 10 wherein said plurality of component fluid streams further comprises at least one component fluid stream at a temperature lower than ambient temperature.

12. The method of claim 1 wherein said plurality of component fluid streams comprises one component fluid stream at a temperature lower than ambient temperature.

13. The method of claim 1, herein said plurality of component fluid streams are air.

14. The method of claim 1 wherein said sample contains nucleic acids.

15. The method of claim 1 wherein said plurality of component fluid streams comprises three component streams wherein one component fluid stream is at a temperature higher than ambient temperature one component fluid stream is at a temperature lower than ambient temperature and one component fluid stream is fluid which has exited from said sample environment and is recirculated into said sample environment.

16. The method of claim 15 wherein said first sample-target-temperature is maintained by recycling said composite stream of step (a) into the environment of said sample.

17. The method of claim 15 wherein said second sample-target-temperature is maintained by recycling the adjusted composite stream of step (c) into the environment of said sample.

18. The method of claim 1 wherein a sequence of said first sample-target-temperature and said second sample-target-temperature is repeatedly achieved.

19. A method for thermal cycling nucleic acids comprising the steps of:

a. directing the flow of a composite air stream into the environment of a sample of nucleic acids to achieve a first nucleic acid target-temperature, wherein said composite air stream is composed of a plurality of component air streams each from a distinct source and each maintained at a different constant temperature;

b. maintaining said first nucleic acid target-temperature for a preselected period;

c. adjusting said composite air stream to achieve a second nucleic acid target-temperature wherein when said first nucleic acid target-temperature is less than said second nucleic acid target-temperature, a sample-ramp-speed of at least 1.0°

C./second is achieved, and wherein said adjusting is achieved by selectively blending in varying ratios said component air streams; and

d. maintaining said second nucleic acid target-temperature for a preselected period.

20. The method of claim 19 wherein said composite air stream of step (a) has a temperature substantially the same as said first nucleic acid target-temperature.

21. The method of claim 19 wherein said composite air stream of step (a) initially has a temperature greater than said first nucleic acid target-temperature and after said sample substantially achieves said first nucleic acid target-temperature, said composite air stream is altered to a temperature substantially the same as said first nucleic acid target-temperature.

22. The method of claim 19 wherein said adjusted composite air stream of step (c) has a temperature substantially the same as said second nucleic acid target-temperature.

23. The method of claim 19 wherein said adjusted composite air stream of step (c) initially has a temperature lower than said second nucleic acid target-temperature and after said sample substantially achieves said second nucleic acid target-temperature, said adjusted composite stream has a temperature substantially the same as said second nucleic acid target-temperature.

24. The method of claim 19 wherein said plurality of component air streams comprises at least one component air stream at a temperature higher than ambient temperature.

25. The method of claim 24 wherein said plurality of component air streams further comprises at least one component air stream at a temperature lower than ambient temperature.

26. The method of claim 19 wherein said plurality of component air streams comprises one component air stream at a temperature lower than ambient temperature.

27. The method of claim 19 wherein said plurality of component air streams comprises three component air streams wherein one component air stream is at a temperature higher than ambient temperature, one component air stream is at a temperature lower than ambient temperature and one component air stream is air which has exited from said sample environment and is recirculated into said sample environment.

28. The method of claim 27 wherein said first nucleic acid target-temperature is maintained by recycling said composite air stream into the environment of said sample of nucleic acids.

29. The method of claim 27 wherein said second sample-target-temperature is maintained by recycling the adjusted composite air stream into the environment of said sample of nucleic acids.

30. The method of claim 19 wherein a sequence of said first nucleic acid target-temperature and said second nucleic acid target-temperature is repeatedly achieved.