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Immunoassays with novel labeled carbamazepine hapten analogues

  • US 5,578,457 A
  • Filed: 08/07/1992
  • Issued: 11/26/1996
  • Est. Priority Date: 08/07/1992
  • Status: Expired due to Term
First Claim
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1. An immunoassay method for carbamazepine comprising:

  • A. contacting a liquid sample, containing carbamazepine, with a labeled carbamazepine analogue in the presence of antibodies for carbamazepine under conditions that promote the formation of carbamazepine/antibody immunocomplexes; and

    B. determining the quantity of the drug in the liquid by measuring bound or unbound labeled drug analogue;

    wherein the labeled carbamazepine analogue 1 has the structure (I);

    ##STR12## R represents hydrogen or lower alkyl of 1 to 6 carbon atoms R1 is alkylene of 1 to 3 carbon atoms sufficient to form a heterocyclic group selected from 1,4-piperazinylene;

    2,5-dimethyl-1,4-piperazinylene;

    1,3-imidazolidinylene, and 1,4-hexahydrodiazepinylene;

    R2, R3, R4, and R5 each independently represent alkylene groups of 2 to 10 carbon atoms, or phenylene;

    R7 is hydrogen or methyl;

    each Z independently represents --O--, --S--, or --NR-- wherein R represents hydrogen or lower alkyl of 1 to 6 carbon atoms;

    LABEL is an enzyme;

    1is 0, 1 or 2;

    m is 1 or 2;

    n is 0, 1, or 2; and

    (i) the sum of carbon and hetero atoms in the linking chain, comprising the atoms included in R2, R3, R4, and R5, excluding the amide group of carbamazepine and the carbonyl group joining the label to the linking chain, is 5 to 40, (ii) provided that the bracketed components 1, m and n of structure I can appear therein in any order, and (iii) only one R2, R3, R4, or R5 group can be phenylene.

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