Assay of water pollutants
First Claim
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1. A method of assay of biological or chemical quality, in terms of antioxidant capacity, of a sample of water suspected to contain pollutants having an antioxidant capacity, which method comprises the steps of:
- (1) carrying out a control, enzyme-catalyzed chemiluminescent action in a reaction mixture containing a chemical species starting material for said control reaction and measuring a level of chemiluminescence thereby generated;
(2) initiating and carrying out the same chemiluminescent reaction in the same reaction mixture to which said sample suspected to contain pollutants having an antioxidant capacity is added prior to or during said reaction, said chemiluminescent reaction generating a level of chemiluminescence whereby, when said sample is polluted, addition of said sample to said chemiluminescent reaction causes a reduction in said level of luminescence compared with the level of chemiluminescence generated by said control chemiluminescence reaction;
(3) monitoring said reduction in level of chemiluminescence, and(4) determining the water quality in terms of antioxidant capacity from said reduced level of chemiluminescence.
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Abstract
A method of assay of antioxidant activity of a sample of water, which comprises monitoring the change exerted by the sample on a luminescent reaction.
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14 Claims
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1. A method of assay of biological or chemical quality, in terms of antioxidant capacity, of a sample of water suspected to contain pollutants having an antioxidant capacity, which method comprises the steps of:
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(1) carrying out a control, enzyme-catalyzed chemiluminescent action in a reaction mixture containing a chemical species starting material for said control reaction and measuring a level of chemiluminescence thereby generated; (2) initiating and carrying out the same chemiluminescent reaction in the same reaction mixture to which said sample suspected to contain pollutants having an antioxidant capacity is added prior to or during said reaction, said chemiluminescent reaction generating a level of chemiluminescence whereby, when said sample is polluted, addition of said sample to said chemiluminescent reaction causes a reduction in said level of luminescence compared with the level of chemiluminescence generated by said control chemiluminescence reaction; (3) monitoring said reduction in level of chemiluminescence, and (4) determining the water quality in terms of antioxidant capacity from said reduced level of chemiluminescence. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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