Topical application of steroid hormones and vaginocervical stimulation to induce maternal behavior of animals

• US 5,582,592 A
• Filed: 05/27/1994
• Issued: 12/10/1996
• Est. Priority Date: 06/25/1991
• Status: Expired due to Fees

• Current Assignee: Ministry of Municipality & Rural Affairs
• Original Assignee: Ministry of Municipality & Rural Affairs
• Inventors: Kendrick, Keith M.
• Primary Examiner(s): Green, Randall L.
• Assistant Examiner(s): ELGART, VANITHA M
• Application Number:
  US08/170,195
• Time in Patent Office: 928 Days
• Field of Search: 604/55, 604/10-15, 604/49, 604/906, 604/285-290, 600/33, 600/35, 424/430-433, 424/DIG. 15, 514/967, 514/899, 514/843, 128/830, 128/898
• US Class Current: 604/515
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 31/565 not substituted in position 17 beta by a carbon atom, e.g. estrane, estradiol
• CPC Class Codes: A61K 31/57 substituted in position 17 beta by a chain of two carbon atoms, e.g. pregnane or progesterone

Abstract

The invention provides a process of artificially inducing a non-pregnant, non-human female animal to act as a foster mother using a non-invasive method which comprises administering a topical application of at least one steroid hormone to the animal for a period of time sufficient that uptake of hormone by the animal acts both (i) to stimulate lactation and (ii) to prime the animal for vaginal and cervical stimulation so that such stimulation causes induction of maternal behavior. The hormone may be selected from either progestogen or oestrogen or a mixture of the two, and may be applied to the animal via an intra-vaginal sponge by a method comprising the steps of inserting a first hormone impregnated sponge into the vagina of a non-pregnant animal for about two to three weeks; removing the first sponge and replacing it with a second hormone impregnated sponge for a further period of time of about two to four weeks. Maternal behavior is induced by vaginal and cervical stimulation.

Claims

1. A process of artificially inducing a non-pregnant, non-human female animal to act as a foster mother using a non-invasive method which comprises:

(i) inserting a first hormone impregnated intravaginal sponge into the vagina of an animal, wherein the first hormone impregnated sponge comprises at least one steroid hormone;

(ii) removing the first hormone impregnated sponge and inserting a second hormone impregnated sponge into the vagina of the animal from step (i), wherein the second hormone impregnated sponge comprises at least one steroid hormone; and

(iii) vaginally and cervically stimulating the animal;

wherein the first and second sponges are inserted for a period of time sufficient to induce lactation and so that the stimulation of step (iii) causes induction of maternal behavior.

2. The process according to claim 1, wherein the steroid hormone is selected from either progestogen or oestrogen or a mixture of the two.

3. The process according to claim 1, wherein the first sponge is impregnated with progesterone and β

-oestradiol in a 10;

1 by weight ratio and the second sponge is impregnated with progesterone and β

-oestradiol in a 5;

1 by weight ratio.

4. The process according to claim 3, wherein the first sponge contains about 250 mg of progesterone and about 25 mg of β

-oestradiol and the second sponge contains about 250 mg of progesterone and about 50 mg of β

-oestradiol.

5. The process according to claim 1, which comprises using a three week treatment with a vaginal sponge or insert containing 250 mg progesterone (or medroxyprogesterone acetate) and 50 mg β

-oestradiol (oestradiol dipropionate) followed by a further three week treatment with a vaginal sponge or insert containing the same concentration of progestagen and double that of progestogen.

6. The process according to claim 5, modified by using 100 mg oestrogen in the first sponge/insert and by using 250 mg oestrogen in the second sponge/insert.

7. The process according to claim 1, wherein steriod hormone treatment is combined with exposure of the animal to 16-20 hours of artificial light per day during a six week treatment period.

8. The process according to claim 1, wherein the non-pregnant animal is multiparous.

9. The process according to claim 1, wherein the non-pregnant animal is nulliparous.

10. The process according to claim 1, wherein the non-pregnant animal is primiparous.

11. The process according to claim 9, wherein the non-pregnant animal is a virgin.

12. The process according to claim 1, wherein the animal is of an ungulate species.

13. The process according to claim 1, wherein the animal is selected from the group consisting of cow, sheep, buffalo, oxen, goat, horse, pig and camel.

14. The process according to claim 1, wherein maternal behavior is induced by vaginal and cervical stimulation.

15. The process according to claim 14, wherein manual vaginal and cervical stimulation is carried out by rhythmically pushing a hand up into the vaginal and exerting pressure on the cervix.

16. The process according to claim 15, wherein the index and middle finger of the hand are extended to enter the neck of the cervix and are used to stretch the neck of the cervix while the remainder of the hand is moved backwards and forwards within the vagina.

17. The process according to claim 14, wherein maternal behavior is induced by vaginal and cervical stimulation using a probe.

18. The process according to claim 14, wherein a juvenile animal to be fostered is smeared with vaginal secretion from the foster mother animal following the vaginal and cervical stimulation.

19. The process of claim 1 wherein the first sponge is inserted for a period of about two to three weeks and the second sponge is inserted for a period of about two to four weeks.