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Process for sealing at least one well out of a number of wells provided in a plate for receiving chemical and/or biochemical and/or microbiological substances, and installation for carrying out the process

  • US 5,582,665 A
  • Filed: 01/25/1995
  • Issued: 12/10/1996
  • Est. Priority Date: 07/18/1990
  • Status: Expired due to Fees
First Claim
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1. A process for sealing at least one well out of a number of wells having openings facing upwards, said wells being provided in a plate means and serving to receive chemical and/or biochemical and/or microbiological substances, the openings having their orifices in the upper side of said plate means, wherein the process comprises the steps of:

  • a) placing said plate means with said wells facing upwards onto a receiving member having means for receiving said wells so that said plate means contacts an upper face of said receiving member,b) placing a heat deformable coverplate from a thermoplastic resin sheet on said upper side of said plate means, at least said opening of said at least one well to be sealed being covered by an area of said coverplate, which coverplate has an upper side facing upwards, and thenc) providing a joining seam running at least around said opening of said well to be sealed and joining at least the area, covering said opening of said well with said coverplate, said joining seam having a profiled cross-section in order to increase the contact surface between said upper side of said plate means and said coverplate, by the following steps;

    ba) providing a welding die member having a front face above said receiving member so that said front face thereof opposes said upper face of said receiving member,bb) providing one of said opposing faces with a substantially flat surface and the other of said opposing faces with projecting profiled regions, wherein at least said other face is heated to a temperature which is in the region of the melting point of, at least, said coverplate,bc) bringing the opposing faces of said welding die member and said receiving member together so that said front face of said welding die member contacts said upper side of said coverplate, said profiled regions coming into contact with one of said upper side of said coverplate or said plate means in the area around said opening;

    thenbd) pressing said welding die member and said receiving member together through a fixed path so that said profiled regions at least penetrate into said coverplate; and

    thend) separating said welding die member from said receiving member.

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