Apparatus and method for electrophoresis

• US 5,582,702 A
• Filed: 04/26/1995
• Issued: 12/10/1996
• Est. Priority Date: 04/26/1995
• Status: Expired due to Term

• Current Assignee: Life Technologies Limited (Thermo Fisher Scientific Incorporated)
• Original Assignee: Ethrog Biotechnology Limited
• Inventors: Cabilly, Shmuel, Yogev, Uri
• Primary Examiner(s): Phasge, Arun S.
• Application Number:
  US08/427,917
• Time in Patent Office: 594 Days
• Field of Search: 204/182.8, 204/299 R, 204/456, 204/465, 204/606, 204/615, 204/612
• US Class Current: 204/456
• CPC Class Codes: B01D 57/02 by electrophoresis treatment of water, waste water, sewage or sludge by electrophoresis C02F1/469; electrophoretic production of compounds or non-metals C25B7/00; investigating or analysing materials by using electrophoresis G01N27/26
• CPC Class Codes: G01N 27/44704 Details; Accessories
• CPC Class Codes: G01N 27/44721 by optical means

Abstract

A substantially closed cassette for conducting therein electrophoresis separation which includes a closed chamber, the chamber includes therein a body of gel, means for providing ions for driving the electrophoresis separation and electrodes for connecting the cassette to an external electrical power source, thereby enabling to drive the electrophoresis separation.

Claims

1. Apparatus for conducting electrophoresis separation therein, the apparatus comprising:

a housing having at least bottom and side walls defining a chamber, said chamber comprises, in contact therebetween;

a body of gel for carrying therein said electrophoresis separation;

at least one body of ion exchange matrix for providing ions for driving said electrophoresis separation; and

electrodes for connecting said chamber to an external electrical power source, thereby enabling to drive said electrophoresis separation.

2. Apparatus according to claim 1 wherein said at least one body of ion exchange matrix comprises:

a body of cation exchange matrix for providing the cations for driving said electrophoresis separation; and

a body of anion exchange matrix for providing the anions for driving said electrophoresis separation.

3. Apparatus according to claim 2 wherein said cation exchange matrix is disposed at one end of said body of gel and said body of anion exchange matrix is disposed on a second end of said gel.

4. Apparatus according to claim 2 wherein said cation exchange matrix exchanges protons derived from electrolysis with the cations for driving said electrophoresis separation and said anion exchange matrix exchanges hydroxyl ions derived from said electrolysis with the anions for driving said electrophoresis separation.

5. Apparatus according to claim 2 wherein said cation exchange matrix and said anion exchange matrix comprises particles immersed in a support matrix.

6. Apparatus according to claim 5 wherein said support matrix comprises said gel.

7. Apparatus according to claim 1 and also comprising a cover for closing said chamber, thereby providing a substantially closed apparatus.

8. Apparatus according to claim 7 wherein said cover have at least one opening therein for introducing a test sample into said body of gel.

9. Apparatus according to claim 8 wherein said openings are closed by a comb structure prior to the electrophoresis separation.

10. Apparatus according to claim 7 wherein at least said cover and said bottom wall are transparent to ultra violet (UV) radiation.

11. Apparatus according to claim 7 and also comprising closed vent holes in said cover, said vent holes being opened when the apparatus is used.

12. Apparatus according to claim 7 wherein said apparatus is substantially flat.

13. Apparatus according to claim 1 and also comprising a buffer solution in contact with said body of gel, said at least one body of ion exchange matrix and said electrodes.

14. Apparatus according to claim 13 wherein said buffer is a TAE buffer, said cation exchange matrix releases Tris cations and said anion exchange matrix releases acetate anions.

15. Apparatus according to claim 14 wherein said buffer also comprises:

ethydium bromide and said cation exchange matrix also releases ethydium cations.

16. Apparatus according to claim 1 wherein said apparatus is disposable.

17. A substantially closed cassette for conducting therein electrophoresis separation comprising:

a closed chamber which comprises therein;

a body of gel for carrying therein said electrophoresis separation;

at least one body of ion exchange matrix for providing ions for driving said electrophoresis separation; and

electrodes for connecting said cassette to an external electrical power source, thereby enabling to drive said electrophoresis separation.

18. A cassette according to claim 17 wherein said chamber having top, bottom and side walls, wherein one of said bottom and top walls is an openable cover.

19. A cassette according to claim 18 wherein said top wall includes at least one openable vent hole.

20. A cassette according to claim 17 wherein one of said bottom and top walls includes openings for introducing a test sample into said body of gel.

21. A cassette according to claim 20 and further comprising a comb structure for closing said openings.

22. A cassette according to claim 17 wherein said means for providing ions comprises at least one ion exchange matrix.

23. A cassette according to claim 17 wherein said cassette is substantially flat.

24. A cassette according to claim 17 wherein said cassette is disposable.

25. A system for conducting electrophoresis separation comprising:

an electrical power source;

a substantially closed disposable cassette for conducting an electrophoresis separation therein and having conductive elements therein, said cassette comprising therein a body of gel for carrying therein said electrophoresis separation and at least one body of ion exchange matrix for providing ions for driving said electrophoresis separation; and

a support for supporting said substantially closed cassette and for connecting said electrical power source to said conductive elements of said cassette.

26. A system according to claim 25 and wherein said system also comprising a UV light source and wherein said cassette is transparent to UV light and comprises a UV sensitive material capable of interacting with the molecules undergoing electrophoresis separation and of emitting light, thereby enabling to conduct said electrophoresis separation and to visualize it while said cassette is in situ.

27. A system according to claim 26 wherein said UV sensitive material is ethydium bromide.

28. A system according to claim 26 wherein said cassette includes therein at least one ion exchange matrix for providing said UV detectable material.

29. A system according to claim 25 and also comprising camera means for documenting the results of said electrophoresis separation.

30. A system according to claim 25 and also comprising a cooling system for cooling said cassette during said electrophoresis test.

31. An electrophoresis method comprising the steps of:

introducing a test sample into a body of gel;

applying an electrical field to said gel; and

driving an electrophoresis separation by providing ions required for driving said electrophoresis separation by at least one ion exchange matrix.

32. A method for producing a substantially closed cassette for conducting electrophoresis separation therein comprising:

providing a housing having bottom and side walls defining an open chamber;

assembling within said chamber in contact therebetween a body of gel for carrying therein said electrophoresis separation, at least one body of ion exchange matrix for providing ions for driving said electrophoresis separation and electrodes for connecting said chamber to an external electrical power source; and

closing said open housing with a cover, thereby forming a substantially closed cassette capable of carrying electrophoresis separation therein.