Electrical method and apparatus for non-contact determination of physical and/or chemical properties of a sample, particularly of blood

• US 5,583,432 A
• Filed: 04/11/1994
• Issued: 12/10/1996
• Est. Priority Date: 04/11/1994
• Status: Expired due to Fees

• Current Assignee: Sci-Nostics Limited
• Original Assignee: Sci-Nostics Limited
• Inventors: Barnes, Christopher
• Primary Examiner(s): Snow, Walter E.
• Application Number:
  US08/226,075
• Time in Patent Office: 974 Days
• Field of Search: 324/204, 324/232, 324/226, 324/262
• US Class Current: 324/204
• CPC Class Codes: G01N 15/05 in blood
• CPC Class Codes: G01N 33/49 Blood chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80

Abstract

A method and apparatus for investigating various parameters of biofluids, such as blood, particularly protein and cellular concentrations and cellular volume utilises the application of at least two a.c. frequencies, preferably simultaneously, in the range 0.1 to 60 MHz to a sample of the fluid and receiving and resolving the frequencies after passage through the fluid. The received frequencies are processed to derive corresponding d.c. voltage amplitudes which are mathematically processed to provide a numeric output correlating with a parameter under investigation, A new parameter named Instant Sedimentation Rate (ISR) is disclosed.

Claims

1. A method for investigating one or more parameters of biofluids, particularly blood in respect of protein and cellular concentrations and cellular volume, comprising the steps of:

applying to a sample of the biofluid and without direct contract with the sample at least two a.c. frequencies in a range of 0.1 to 60 MHz;

simultaneously receiving and resolving said frequencies after their passage through said fluid;

obtaining subsequent d.c. voltage amplitudes by detection of said received and resolved frequencies;

thereafter applying mathematical processing to said d.c. voltage amplitudes to provide a numeric output which correlates with at least one of said parameters.

2. A method as claimed in claim 1, wherein said two frequencies form a pair within a recognised frequency dispersion, and at least one of the frequencies is close to a maximum amplitude of said dispersion.

3. A method as claimed in claim 2, wherein both of said frequencies offset to a same side of the peak frequency.

4. A method as claimed in claim 2, wherein the biofluid is blood and the pair of frequencies is within a beta dispersion, the parameter under determination being the mean cell volume (MCV).

5. A method as claimed in claim 4, wherein the pair of frequencies is on a low frequency side of a dispersion peak frequency.

6. A method according to claim 5, wherein the frequency pair is in a range of 1.5 to 2.6 MHz.

7. A method as claimed in claim 2, wherein the biofluid is blood and the pair of frequencies is within a beta-1 dispersion, the parameter under determination being haemoglobin level (Hb).

8. A method according to claim 7, wherein the pair of frequencies is on a high frequency side of a dispersion peak frequency.

9. A method according to claim 8, wherein the frequency pair is within a range of 14 to 22 MHz.

10. A method as claimed in claim 2, wherein the biofluid is blood and the pair of frequencies is within a delta dispersion, the parameter undergoing determination being the total protein content.

11. A method as claimed in claim 10, wherein fibrinogen level is determined by subtracting haemoglobin level from a total protein content value.

12. A method as claimed in claim 11, wherein the pair of frequencies is within a beta -1 dispersion which is used to determine haemoglobin level.

13. A method as claimed in claim 11, wherein the haemoglobin level is determined separately and inputted manually.

14. A method as claimed in claim 11, wherein the parameter under determination is instantaneous sedimentation rate (ISR).

15. A method as claimed in claim 2, wherein said mathematical processing includes a division of one of said d.c. voltage amplitudes by the other of said d.c. voltage amplitudes.

16. A method as claimed in claim 2, wherein said mathematical processing includes a subtraction step in respect of one of said parameters.

17. A method as claimed in claim 1, wherein the biofluid is blood and said one or more parameters are chosen from haemoglobin content, red cell count, mean cell volume, total protein content, fibrinogen level and fibrinogen level scaled in terms of instant sedimentation rate (ISR).

18. A method as claimed in claim 1, wherein said a.c. frequencies are each stable and of non-varying amplitude and frequency.

19. An apparatus for obtaining a numeric output which correlates with at least one or more parameters of a biofluid, said parameters including protein and cellular concentrations and cellular volume of blood, the apparatus comprising:

a measurement cell for containing a sample of a biofluid;

means for applying a signal having at least two a.c. frequencies in a range of 0.1 to 60 MHz to the said sample of the biofluid within said cell and without direct contact with the sample;

means for receiving and resolving said signal having at least two a.c. frequencies after passage through said sample of biofluid;

means for obtaining d.c. voltage amplitudes by detection of said received and resolved signal having at least two a.c. frequencies; and

means for applying a mathematical processing to said obtained d.c. voltage amplitudes to provide said numeric output which correlates with at least one of said one or more parameters of the biofluid.

20. Apparatus according to claim 19 wherein, said means for applying and receiving said two or more frequencies are arranged externally of said cell.

21. Apparatus as claimed in claim 19, wherein said means for applying and receiving said two or more frequencies are disposed within a probe which inserted into a sample in said cell.