Oral administration of chicken yolk immunoglobulins to lower somatic cell count in the milk of lactating ruminants

• US 5,585,098 A
• Filed: 01/06/1995
• Issued: 12/17/1996
• Est. Priority Date: 11/23/1993
• Status: Expired due to Term

• Current Assignee: Mueller and Smith
• Original Assignee: Ovimmune, Inc.
• Inventors: Coleman, Marilyn A.
• Primary Examiner(s): Schwadron, Ronald B.
• Application Number:
  US08/369,310
• Time in Patent Office: 711 Days
• Field of Search: 424/804, 424/802, 424/803, 424/823, 424/824, 424/825, 424/150.1, 424/157.1, 424/163.1, 424/164.1, 424/165.1, 424/168.1, 424/169.1, 424/170.1 , 424/151.1, 424/166.1, 530/388.4, 530/388.6, 530/389.5, 530/390.1, 530/861, 530/388.5, 935/107
• US Class Current: 424/157.1
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 39/085 Staphylococcus
• CPC Class Codes: A61K 39/092 Streptococcus
• CPC Class Codes: C07K 16/02 from eggs
• CPC Class Codes: C07K 16/1271 from Micrococcaceae (F), e.g. Staphylococcus
• CPC Class Codes: C07K 16/1275 from Streptococcus (G)
• CPC Class Codes: Y10S 424/802 Antibody or antigen-binding fragment thereof that binds gram-positive bacteria
• CPC Class Codes: Y10S 424/803 Antibody or antigen-binding fragment thereof that binds gram-negative bacteria
• CPC Class Codes: Y10S 424/804 involving IgG3, IgG4, IgA, or IgY
• CPC Class Codes: Y10S 530/861 involving IgG3, IgG4, IgA, or IgY

Abstract

A method for lowering somatic cell count in the milk of a lactating ruminant is disclosed. IgY antibodies are first obtained from the egg of a hen which has been actively immunized against one or more mastitis-causing pathogenic organisms by injection with an immunogen containing immunogenic determinants specific to elicit such antibodies. The immunogenic determinant may comprise only a specific portion of the pathogenic organism, e.g., the fimbria of a piliated bacterium. The IgY antibodies are then administered orally to a ruminant in which it is desired to lower milk somatic cell count. Antibody administration may occur during a ruminant'"'"'s dry period as well as during lactation. In a preferred embodiment, the antigen used in immunization of the hen comprises one or more of Staphylococcus aureus and Streptococcus agalactiae. The method of this invention has been shown to be efficacious in lowering somatic cell count in dairy cattle.

Claims

1. A method for lowering somatic cell count in the milk of a lactating ruminant infected with a mastitis-causing organism, which comprises the steps of:

(a) injecting a fowl hen with an immunogen containing immunogenic determinants specific to elicit IgY antibodies against said mastitis-causing organism; and

(b) orally administering said IgY antibodies to said infected lactating ruminant.

2. The method of claim 1, wherein said mastitis-causing organism is selected from the group consisting of Staphylococcus aureus, Streptococcus agalactiae, Escheria coli, Corynebacterium (Actinomyces) pyogenes, Mycoplasma bovis, Pseudomonas pyocyaneus, Streptococcus uberis, Streptococcus dysgalactiae, Mycobacterium bovis, Klebsiella spp., Serratia marcescens, Nocardia asteroides, Proteus spp., and Chlamydia psittaci.

3. The method of claim 2, wherein said mastitis-causing organism is selected from the group consisting of Staphylococcus aureus, Streptococcus agalactiae, Escheria coli, Corynebacterium (Actinomyces) pyogenes, and Mycoplasma bovis.

4. The method of claim 1, wherein said lactating ruminant is selected from the group consisting of dairy cattle, milking goats, beef cattle, sheep, deer, milking buffalo, and yaks.

5. The method of claim 4, wherein said lactating ruminant is selected from the group consisting of dairy cattle and milking goats.

6. The method of claim 1, wherein said fowl hen is selected from the group consisting of chicken, duck, goose, and turkey.

7. The method of claim 6, wherein said fowl hen is a chicken.

8. The method of claim 1, wherein said IgY antibodies are obtained from the yolk of an egg without fractionation thereof.

9. The method of claim 8, wherein the IgY antibodies are in the form of a powder obtained by stirring the yolk of said egg into an emulsion and drying the emulsion to form a powder.

10. The method of claim 5, wherein said mastitis-causing organism is selected from the group consisting of Staphylococcus aureus and Streptococcus agalactiae.

11. The method of claim 1, wherein the immunization of said fowl hen is performed by administration of the immunogen in conjunction with a water-in-oil emulsion adjuvant.

12. A method for treating mastitis, as determined by measurement of somatic cell count in a ruminant, caused or exacerbated by one or more pathogenic organisms in said ruminant, which comprises the steps of:

(a) injecting a fowl hen with an immunogen containing immunogenic determinants specific to elicit IgY antibodies against said one or more pathogenic organisms; and

(b) orally administering said IgY antibodies to said ruminant.

13. The method of claim 12, wherein said one or more pathogenic organisms is selected from the group consisting of Staphylococcus aureus, Streptococcus agalactiae, Escheria coli, Corynebacterium (Actinomyces) pyogenes, Mycoplasma bovis, Pseudomonas pyocyaneus, Streptococcus uberis, Streptococcus dysgalactiae, Mycobacterium bovis, Klebsiella spp., Serratia marcescens, Nocardia asteroides, Proteus spp., and Chlamydia.

14. The method of claim 13, wherein said one or more pathogenic organisms is selected from the group consisting of Staphylococcus aureus, Streptococcus agalactiae, Escheria coli, Corynebacterium (Actinomyces) pyogenes, and Mycoplasma bovis.

15. The method of claim 12, wherein said ruminant is selected from the group consisting of dairy cattle and milking goats.

16. The method of claim 15, wherein said fowl hen is a chicken.

17. The method of claim 12, wherein said IgY antibodies are obtained from the yolk of an egg without fractionation thereof.

18. The method of claim 12, wherein the IgY antibodies are in the form of a powder obtained by stirring the yolk of an egg into an emulsion and drying the emulsion to form a powder.

19. The method of claim 16, wherein said one or more pathogenic organisms is selected from the group consisting of Staphylococcus aureus and Streptococcus agalactiae.

20. The method of claim 12, wherein the immunization of said fowl hen is performed by administration of the immunogen in conjunction with a water-in-oil emulsion adjuvant.