Method of preparing gas and gaseous precursor-filled microspheres
First Claim
1. Gaseous precursor-filled liposomes prepared by a gel state shaking gaseous precursor instillation method, said method comprising adding a lipid to an aqueous solution or suspension, shaking said lipid-containing aqueous solution or suspension in the presence of a temperature activated gaseous precursor at a temperature below the gel state to liquid crystalline state phase transition temperature of the lipid, wherein said gaseous precursor is a fluorine-containing gaseous precursor, said fluorine-containing gaseous precursor selected from the group consisting of perfluorocarbons, fluorohydrocarbons, and sulfur hexafluoride, wherein upon activation of said gaseous precursor to a gas said liposomes have at least about a 90% volume of gas in the interior thereof.
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Abstract
Methods of and apparatus for preparing temperature activated gaseous precursor-filled liposomes are described. Gaseous precursor-filled liposomes prepared by these methods are particularly useful, for example, in ultrasonic imaging applications and in therapeutic drug delivery systems.
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Citations
21 Claims
- 1. Gaseous precursor-filled liposomes prepared by a gel state shaking gaseous precursor instillation method, said method comprising adding a lipid to an aqueous solution or suspension, shaking said lipid-containing aqueous solution or suspension in the presence of a temperature activated gaseous precursor at a temperature below the gel state to liquid crystalline state phase transition temperature of the lipid, wherein said gaseous precursor is a fluorine-containing gaseous precursor, said fluorine-containing gaseous precursor selected from the group consisting of perfluorocarbons, fluorohydrocarbons, and sulfur hexafluoride, wherein upon activation of said gaseous precursor to a gas said liposomes have at least about a 90% volume of gas in the interior thereof.
- 2. Gas-filled liposomes prepared by a gel state shaking gas instillation method, said liposomes comprising a lipid and a fluorine-containing gas, said fluorine-containing gas selected from the group consisting of perfluorocarbons, fluorohydrocarbons, and sulfur hexafluoride, said liposomes prepared by a method comprising adding a lipid to an aqueous solution or suspension, shaking said lipid-containing aqueous solution or suspension in the presence of a temperature activated gaseous precursor at a temperature below the gel state to liquid crystalline state phase transition temperature of the lipid, said liposomes having at least about a 90% volume of gas in the interior thereof.
Specification