Cellular attachment to trans-epithelial appliances

• US 5,585,267 A
• Filed: 10/03/1994
• Issued: 12/17/1996
• Est. Priority Date: 04/05/1993
• Status: Expired

• Current Assignee: Desmos, Inc.
• Original Assignee: Desmos, Inc.
• Inventors: Jones, Jonathan, Quaranta, Vito, Tamura, Richard
• Primary Examiner(s): NOT, DEFINED
• Assistant Examiner(s): Larson, Kristin
• Application Number:
  US08/317,223
• Time in Patent Office: 806 Days
• Field of Search: 435/240.2, 435/240.243, 435/240.23, 435/283.1, 606/1, 606/151, 606/167
• US Class Current: 435/402
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61L 27/227 Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
• CPC Class Codes: A61L 27/34 Macromolecular materials
• CPC Class Codes: A61L 27/3633 Extracellular matrix [ECM]
• CPC Class Codes: A61L 27/38 containing added animal cells organs or tissue containing native cells A61L27/36
• CPC Class Codes: A61L 27/3813 Epithelial cells, e.g. keratinocytes, urothelial cells
• CPC Class Codes: A61L 27/3869 Epithelial tissues other than skin
• CPC Class Codes: A61L 27/60 Materials for use in artificial skin
• CPC Class Codes: A61L 29/085 Macromolecular materials
• CPC Class Codes: A61L 31/10 Macromolecular materials
• CPC Class Codes: C07K 14/4705 stimulating, promoting or activating activity
• CPC Class Codes: C07K 14/78 Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
• CPC Class Codes: C07K 16/18 against material from animals or humans
• CPC Class Codes: C08L 89/00 Compositions of proteins; Compositions of derivatives thereof foodstuff preparations A23J3/00
• CPC Class Codes: C12N 2502/253 Bladder cells
• CPC Class Codes: C12N 2533/90 Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: C12N 5/0621 Eye cells, e.g. cornea, iris pigmented cells photoreceptors C12N5/062
• CPC Class Codes: C12N 5/0629 Keratinocytes; Whole skin
• CPC Class Codes: C12N 5/0676 Pancreatic cells

Abstract

A trans-epithelial appliance having a hemidesmosome formation-inducing protein composition derived from rat bladder carcinoma cells deposited thereon. This composition stimulates cell attachment and may be either the cell matrix or a soluble factor isolated from the conditioned medium. The appliance will be useful for diminishing inflammation and/or infection at the site of entry of the appliance. The appliance may also be used to stimulate gum junctional epithelium adhesion in the treatment of gingivitis and periodontitis. The composition may be used to maintain tissues ex vivo.

Claims

1. An article of manufacture comprising a trans-epithelial appliance coated with a soluble hemidesmosome formation-inducing factor obtainable from 804G or NBT-II rat bladder carcinoma cells, wherein the factor induces epithelial cell attachment to the coated trans-epithelial appliance.

2. The article of claim 1, wherein said appliance is a shaped article selected from the group consisting of indwelling catheter, needle, metal pin, metal rod, colostomy tube, dental abutment piece and surgical mesh.

3. The article of claim 1, further comprising epithelial cells deposited on said hemidesmosome formation-inducing composition.

4. The article of claim 1, wherein said appliance is used in vivo.

5. The article of claim 1, wherein said appliance is made of or coated with a biocompatible metal.

6. The article of claim 5, wherein said metal is stainless steel or titanium.

7. The article of claim 1, wherein said appliance is made of or coated with a ceramic material.

8. The article of claim 7, wherein said material is hydroxyapatite.

9. The article of claim 1, wherein said appliance is made of or coated with a polymer.

10. The article of claim 9, wherein said polymer is selected from the group consisting of polyester, polyglycolic acid and a polygalactose-polyglycolic acid copolymer.

11. A method for inducing epithelial cell attachment to a trans-epithelial appliance comprising the following steps:

(a) coating a trans-epithelial appliance with a soluble hemidesmosome formation-inducing factor obtainable from 804G or NBT-II rat bladder carcinoma cells, and

(b) incubating the coated trans-epithelial appliance with epithelial cells under conditions necessary to induce epithelial cell attachment to the coated trans-epithelial appliance.

12. The method of claim 11, wherein said appliance is selected from the group consisting of indwelling catheter, needle, metal pin, metal rod, colostomy tube, dental abutment piece and surgical mesh.

13. The method of claim 11, wherein said appliance is made of or coated with a polymer.

14. The method of claim 13, wherein said polymer is selected from the group consisting of polyester, polyglycolic acid and a polygalactose-polyglycolic acid copolymer.