Time-resolved luminescence binding assays using a fluorescent transition metal label other than ruthenium
First Claim
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1. A time-resolved luminescence immunoassay method for detection of an analyte involving a metal-labelled binding partner, comprising the steps of:
- a) binding the analyte to an immobilized anti-analyte first binding partner;
b) adding fluorescent metal-labelled second binding partner specific for the analyte, where the metal label comprises a luminescent transition metal complex, provided the transition metal is not ruthenium;
c) washing off the unbound labelled binding partner;
d) eluting the analyte-first binding partner-second fluorescent binding partner complex, and determining the fluorescence of the metal label by time-resolved fluorescence spectroscopy, thereby detecting the analyte.
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Abstract
A time-resolved luminescence binding assay is described including a metal-labelled binding partner comprising a luminescent transition metal complex covalently attached to a binding partner such as an antigen or antibody. The metal-labelled binding partner, and a process for preparing said metal-labelled binding partner are described.
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Citations
6 Claims
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1. A time-resolved luminescence immunoassay method for detection of an analyte involving a metal-labelled binding partner, comprising the steps of:
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a) binding the analyte to an immobilized anti-analyte first binding partner; b) adding fluorescent metal-labelled second binding partner specific for the analyte, where the metal label comprises a luminescent transition metal complex, provided the transition metal is not ruthenium; c) washing off the unbound labelled binding partner; d) eluting the analyte-first binding partner-second fluorescent binding partner complex, and determining the fluorescence of the metal label by time-resolved fluorescence spectroscopy, thereby detecting the analyte. - View Dependent Claims (2, 3, 4, 5, 6)
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Specification