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Time-resolved luminescence binding assays using a fluorescent transition metal label other than ruthenium

  • US 5,585,279 A
  • Filed: 08/08/1994
  • Issued: 12/17/1996
  • Est. Priority Date: 01/23/1986
  • Status: Expired due to Term
First Claim
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1. A time-resolved luminescence immunoassay method for detection of an analyte involving a metal-labelled binding partner, comprising the steps of:

  • a) binding the analyte to an immobilized anti-analyte first binding partner;

    b) adding fluorescent metal-labelled second binding partner specific for the analyte, where the metal label comprises a luminescent transition metal complex, provided the transition metal is not ruthenium;

    c) washing off the unbound labelled binding partner;

    d) eluting the analyte-first binding partner-second fluorescent binding partner complex, and determining the fluorescence of the metal label by time-resolved fluorescence spectroscopy, thereby detecting the analyte.

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