Mesoscale polynucleotide amplification devices

• US 5,587,128 A
• Filed: 11/14/1994
• Issued: 12/24/1996
• Est. Priority Date: 05/01/1992
• Status: Expired due to Term

• Current Assignee: Trustees Of The University Of Pennsylvania (University of Pennsylvania)
• Original Assignee: Trustees Of The University Of Pennsylvania (University of Pennsylvania)
• Inventors: Kricka, Larry J., Wilding, Peter
• Primary Examiner(s): Marschel, Ardin H.
• Application Number:
  US08/338,728
• Time in Patent Office: 771 Days
• Field of Search: 422/50, 422/54, 422/55, 422/56, 422/57, 422/58, 422/68.1, 422/69, 422/82.01, 422/82.02, 422/82.05-.09, 422/129, 422/131, 422/138, 435/91.2, 435/91.1, 435/6, 435/5, 435/291, 435/810, 435/90, 435/91, 435/91.3, 435/91.5, 435/91.51, 435/173.1, 435/174, 435/176, 435/177, 435/283.1, 435/285.1 , 435/285.2, 435/287.1-.3, 435/287.7-.9, 435/288.7, 435/289.1, 435/290.1, 435/292.1, 435/299.1, 435/808, 435/814, 437/1, 437/5, 437/51, 437/61, 437/180, 437/181, 437/189, 437/225, 437/946, 536/22.1, 536/23.1, 536/25.3, 935/77, 935/78, 935/88, 436/518, 436/524, 436/525, 436/528, 436/531, 436/532, 436/535, 436/63, 436/164-6, 436/169, 436/172, 436/175, 436/805
• US Class Current: 422/50
• CPC Class Codes: B01D 61/18 Apparatus therefor
• CPC Class Codes: B01D 67/0058 by selective elimination of components, e.g. by leaching
• CPC Class Codes: B01D 67/0072 by deposition from the gaseous phase, e.g. sputtering, CVD, PVD
• CPC Class Codes: B01D 71/02 Inorganic material
• CPC Class Codes: B01F 25/41 Mixers of the fractal type
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 19/0093 Microreactors, e.g. miniaturised or microfabricated reactors laboratory containers with capillary fluid transport in microfabricated channels or chambers B01L3/5027
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/146 Employing pressure sensors
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 2300/1861 using radiation
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502707 : characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502715 : characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502753 : characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation separation in general B01D; microapparatus for analysis using electrophoresis G01N27/44791; sample preparation G01N1/28
• CPC Class Codes: B01L 7/52 : with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: B01L 7/525 : with physical movement of samples between temperature zones
• CPC Class Codes: B01L 9/527 : for microfluidic devices, e.g. used for lab-on-a-chip
• CPC Class Codes: C12M 41/46 : of cellular or enzymatic activity or functionality, e.g. cell viability
• CPC Class Codes: C12Q 1/6844 : Nucleic acid amplification reactions
• CPC Class Codes: C12Q 1/686 : Polymerase chain reaction [PCR]
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: Y10S 366/03 : Micromixers: variable geometry from the pathway influences mixing/agitation of non-laminar fluid flow
• CPC Class Codes: Y10S 435/808 : Optical sensing apparatus
• CPC Class Codes: Y10S 435/81 : Packaged device or kit
• CPC Class Codes: Y10S 435/814 : Enzyme separation or purification
• CPC Class Codes: Y10T 436/25125 : Digestion or removing interfering materials

Abstract

Disclosed are devices for amplifying a preselected polynucleotide in a sample by conducting a polynucleotide amplification reaction. The devices are provided with a substrate microfabricated to include a polynucleotide amplification reaction chamber, having at least one cross-sectional dimension of about 0.1 to 1000 μm. The device also includes at least one port in fluid communication with the reaction chamber, for introducing a sample to the chamber, for venting the chamber when necessary, and, optionally, for removing products or waste material from the device. The reaction chamber may be provided with reagents required for amplification of a preselected polynucleotide. The device also may include means for thermally regulating the contents of the reaction chamber, to amplify a preselected polynucleotide. Preferably, the reaction chamber is fabricated with a high surface to volume ratio, to facilitate thermal regulation. The amplification reaction chamber also may be provided with a composition which diminishes inhibition of the amplification reaction by material comprising a wall of the reaction chamber, when such treatment is required.

Claims

1. A device for amplifying a polynucleotide in a sample by conducting a polynucleotide amplification reaction, the device comprising:

a solid substrate fabricated to include at least one polynucleotide amplification reaction chamber, said chamber being permanently sealed with a cover and having one mesoscale cross-sectional dimension of width or depth between about 0.1 and 1,000 μ

m, said reaction chamber comprising at least one wall having associated therewith a composition which diminishes inhibition by said wall of a polynucleotide amplification reaction in said chamber; and

at least one port in fluid communication with said reaction chamber, for introducing said sample into said reaction chamber, said reaction chamber and at least one said port being of dissimilar cross-section.

2. The device of claim 1, which further comprises a flow channel connecting said port with said reaction chamber.

3. The device of claim 1, wherein said solid substrate comprises a material selected from the group consisting of glass, silicon, silica, polysilicon, silicon nitride, silicon dioxide, plastic and organic polymeric material.

4. The device of claim 1, wherein said reaction chamber has a depth less than about 500 μ

m.

5. The device of claim 1, wherein said reaction chamber has a depth less than about 300 μ

m.

6. The device of claim 1, wherein said reaction chamber has a depth less than about 80 μ

m.

7. The device of claim 1, wherein said reaction chamber has a ratio of surface area to volume greater than about 3 mm² /μ

l.

8. The device of claim 7 wherein said ratio is greater than about 5 mm² /μ

l.

9. The device of claim 7 wherein said ratio is greater than about 10 mm² /μ

l.

10. The device of claim 1, wherein said composition is adhered to the surface of said wall.

11. The device of claim 10, wherein said composition is covalently attached to said wall surface.

12. The device of claim 10 wherein said composition comprises a polymer.

13. The device of claim 12 wherein said polymer comprises poly(vinyl chloride).

14. The device of claim 1 wherein said composition comprises a blocking agent in a solution disposed within said reaction chamber.

15. The device of claim 14, wherein said blocking agent is selected from the group consisting of polynucleotides and polypeptides.

16. The device of claim 15 wherein said blocking agent is a blocking polynucleotide selected from the group consisting of DNA, RNA, polyguanylic acid and polyadenylic acid.

17. The device of claim 16 wherein said solution comprising said blocking polynucleotide also comprises a sample polynucleotide to be amplified in said reaction chamber.

18. The device of claim 1 wherein said substrate comprises silicon.

19. The device of claim 18 wherein said composition comprises a silane coating on said wall surface.

20. The device of claim 19 wherein said coating is formed by a reaction of said wall surface with a silane selected from the group consisting of dimethylchlorosilane, dimethyldichlorosilane, hexamethyldisilazane and trimethylchlorosilane.

21. The device of claim 18 wherein said composition comprises a silicone coating on said wall surface.

22. The device of claim 21 wherein said coating is formed by the interaction of said wall surface with a siliconizing reagent.

23. The device of claim 21 further comprising a macromolecule associated with said silicone coating.

24. The device of claim 23 wherein said macromolecule is an amino acid polymer.

25. The device of claim 23 wherein said macromolecule is selected from the group consisting of polyvinylpyrrolidone, polyadenylic acid and polymaleimide.

26. The device of claim 18 wherein said composition comprises a silicon oxide film disposed on said wall surface.

27. The device of claim 1, which further comprises a thermal regulator for regulating temperature within said reaction chamber.

28. The device of claim 1, which further comprises a detection system for detecting a product of said polynucleotide amplification reaction.

29. The device of claim 28, wherein said detection system comprises:

a complex-forming agent which, upon contact with said product of said polynucleotide amplification reaction, forms a detectable complex with said product;

a chamber in which said polynucleotide amplification reaction product is contacted with said complex-forming agent, thereby forming said detectable complex; and

a detector for determining the presence or amount of said detectable complex in said chamber.

30. The device of claim 29, wherein said chamber in which said detectable complex is formed is the polynucleotide amplification reaction chamber.

31. The device of claim 29, wherein said chamber in which said detectable complex is formed is a detection chamber in fluid communication with said polynucleotide amplification reaction chamber.

32. The device of claim 1, wherein said reaction chamber is at least partially covered by said cover disposed over said substrate.

33. The device of claim 2, wherein said port and said flow channel are fabricated in said substrate.

34. The device of claim 2, wherein said port and said flow channel are fabricated in said cover.

35. The device of claim 32 wherein said cover comprises a material selected from the group consisting of glasses and organic polymeric materials.

36. The device of claim 33, further comprising a member extending from said substrate, and capable of sealing said port upon depression of said member against said port.