Mesoscale polynucleotide amplification devices
DCFirst Claim
1. A device for amplifying a polynucleotide in a sample by conducting a polynucleotide amplification reaction, the device comprising:
- a solid substrate fabricated to include at least one polynucleotide amplification reaction chamber, said chamber being permanently sealed with a cover and having one mesoscale cross-sectional dimension of width or depth between about 0.1 and 1,000 μ
m, said reaction chamber comprising at least one wall having associated therewith a composition which diminishes inhibition by said wall of a polynucleotide amplification reaction in said chamber; and
at least one port in fluid communication with said reaction chamber, for introducing said sample into said reaction chamber, said reaction chamber and at least one said port being of dissimilar cross-section.
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Abstract
Disclosed are devices for amplifying a preselected polynucleotide in a sample by conducting a polynucleotide amplification reaction. The devices are provided with a substrate microfabricated to include a polynucleotide amplification reaction chamber, having at least one cross-sectional dimension of about 0.1 to 1000 μm. The device also includes at least one port in fluid communication with the reaction chamber, for introducing a sample to the chamber, for venting the chamber when necessary, and, optionally, for removing products or waste material from the device. The reaction chamber may be provided with reagents required for amplification of a preselected polynucleotide. The device also may include means for thermally regulating the contents of the reaction chamber, to amplify a preselected polynucleotide. Preferably, the reaction chamber is fabricated with a high surface to volume ratio, to facilitate thermal regulation. The amplification reaction chamber also may be provided with a composition which diminishes inhibition of the amplification reaction by material comprising a wall of the reaction chamber, when such treatment is required.
1246 Citations
36 Claims
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1. A device for amplifying a polynucleotide in a sample by conducting a polynucleotide amplification reaction, the device comprising:
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a solid substrate fabricated to include at least one polynucleotide amplification reaction chamber, said chamber being permanently sealed with a cover and having one mesoscale cross-sectional dimension of width or depth between about 0.1 and 1,000 μ
m, said reaction chamber comprising at least one wall having associated therewith a composition which diminishes inhibition by said wall of a polynucleotide amplification reaction in said chamber; andat least one port in fluid communication with said reaction chamber, for introducing said sample into said reaction chamber, said reaction chamber and at least one said port being of dissimilar cross-section. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36)
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Specification