Testing device for liquid and liquid suspended samples

• US 5,589,350 A
• Filed: 01/11/1996
• Issued: 12/31/1996
• Est. Priority Date: 11/06/1992
• Status: Expired due to Term

• Current Assignee: Biolog, Inc.
• Original Assignee: Biolog, Inc.
• Inventors: Bochner, Barry
• Primary Examiner(s): McMahon, Timothy
• Assistant Examiner(s): Carpenter, Robert
• Application Number:
  US08/584,989
• Time in Patent Office: 355 Days
• Field of Search: 435/29, 435/287.7, 435/288.5, 422/99, 422/101, 422/102, 436/178
• US Class Current: 435/29
• CPC Class Codes: B01L 2200/0642 Filling fluids into wells by specific techniques
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: G01N 2035/00148 Test cards, e.g. Biomerieux or McDonnel multiwell test cards

Abstract

Testing of samples in a small, closed, and easy-to-fill device. Simultaneous filling of a large number of test wells using a novel gas-venting design, allowing for multiparameter testing and/or identification of bacteria.

Claims

1. A method for testing samples comprising:

(a) providing a sample in liquid form;

(b) providing a sample delivering means;

(c) providing a device comprising;

i) a housing, ii) a testing region comprising a plurality of test wells contained within said housing, iii) a liquid receiving means at an external surface of said housing, iv) a liquid flow-directing means providing liquid communication between said test wells of said testing region and said liquid receiving means, and v) a single gas-venting, liquid barrier having an inner and outer surface wherein said inner surface is in fluidic communication with said test wells of said testing region;

(d) delivering, internal to said housing, said sample via said sample delivering means to said testing region, such that said liquid enters said device at said liquid receiving means under positive pressure and the air in said device is vented through said gas-venting liquid barrier, wherein said positive pressure is greater than 1 pound per square inch; and

(e) attaching a non-venting material to said outer surface of said gas-venting barrier, such that said device is sealed.

2. The method of claim 1 further comprising, prior to step (d), prefilling said test wells with a test formula.

3. The method of claim 2 wherein said test formula comprises basal medium, one or more carbon sources, and an indicator.

4. The method of claim 3 wherein said indicator is a redox indicator.

5. The method of claim 4 wherein said sample is microbiological.

6. The method of claim 1 wherein said liquid receiving means comprises a liquid entry port at an external surface of said housing.

7. The method of claim 6 wherein said sample delivering means comprises a pipette adapted to fit said liquid entry port.

8. The method of claim 7 wherein said port has internal grooves and said pipette has corresponding threads so that a substantially liquid-tight seal is formed.