Fluorescence detection apparatus

• US 5,589,351 A
• Filed: 12/06/1994
• Issued: 12/31/1996
• Est. Priority Date: 12/06/1994
• Status: Expired due to Fees

• Current Assignee: NPS Pharmaceuticals Incorporated (Shire plc)
• Original Assignee: NPS Pharmaceuticals Incorporated (Shire plc)
• Inventors: Harootunian, Alec T.
• Primary Examiner(s): Gitomer, Ralph J.
• Application Number:
  US08/350,232
• Time in Patent Office: 756 Days
• Field of Search: 435/29, 435/291, 435/808, 422/52, 422/63, 250/328, 250/461.2, 356/246, 356/317-320, 356/417
• US Class Current: 435/29
• CPC Class Codes: C12M 41/46 of cellular or enzymatic activity or functionality, e.g. cell viability
• CPC Class Codes: G01N 2021/6463 Optics
• CPC Class Codes: G01N 2021/6484 Optical fibres
• CPC Class Codes: G01N 2021/6493 by alternating fluorescence/transmission or fluorescence/reflection
• CPC Class Codes: G01N 21/251 Colorimeters; Construction thereof
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 35/0099 comprising robots or similar manipulators robots per se B25J
• CPC Class Codes: G01T 7/02 Collecting means for receiving or storing samples to be investigated and possibly directly transporting the samples to the measuring arrangement; particularly for investigating radioactive fluids sampling, preparing specimens for investigation in general G01N1/00, G01N1/02; shielded cells or rooms structurally combined with manipulatin devices G21F; measuring of chromatographically separated samples G01N30/00 - G01N30/96

Abstract

A fluorescence analysis system detects light transmitted from wells in a sample plate. One embodiment of the system includes a single light detector. A reflector is capable of receiving light from any one of several detection emitters and of reflecting the light toward the light detector. One reflector includes a prism which is mounted on a stepping motor. The prism is selectively rotated so that it faces each detection emitter in turn. The light from several wells in a row is directed toward the light emitter by rotating the prism to face, one-by-one, each of the emitters corresponding to the wells. The detection emitters include one end of an optical fiber bundle. The other end of each bundle is positioned near the wells, with each bundle positioned by a different well to carry light from that well to the corresponding emitter. A collimating lens is positioned between each detection emitter and the rotating prism to focus light from the emitter onto the prism. A baffle is positioned between the light detector and the prism. Light from a xenon arc excitation lamp is carried by optical fiber bundles toward the sample wells. A computerized controller coordinates light detection with the activities of an automated liquid handling system.

Claims

1. An apparatus for detecting light transmitted from a composition disposed in one of a plurality of sample wells, said apparatus comprising:

a single light detector;

a reflector comprising means for selectively receiving light from any one of a plurality of detection emitters positioned about said reflector and of reflecting light toward said light detector; and

a plurality of detection carriers, each of said detection carriers comprising means for carrying light from one of the wells to one of said detection emitters.

2. The apparatus of claim 1, wherein said light detector comprises a photomultiplier tube.

3. The apparatus of claim 1, wherein said reflector comprises a prism.

4. The apparatus of claim 3, wherein said prism is selectively rotatable between a plurality of predetermined positions, said prism having in each position means for receiving light from one of said detection emitters and of reflecting the light toward said light detector.

5. The apparatus of claim 3, wherein said reflector further comprises a plurality of light barriers comprising means for substantially barring said prism from receiving light from more than one of said detection emitters at a time during operation of said apparatus.

6. The apparatus of claim 1, wherein each of said detection carriers comprises a fiber bundle.

7. The apparatus of claim 1, further comprising a plurality of lenses, each of said lenses being positioned between one of said detection emitters and said reflector.

8. The apparatus of claim 1, further comprising:

an excitation light source; and

a plurality of excitation carriers, each of said excitation carriers comprising means for carrying light from said excitation light source toward one of the wells.

9. An apparatus for detecting light transmitted from a composition disposed in one of a plurality of sample wells, said apparatus comprising:

a single photomultiplier tube;

a prism comprising means for selectively receiving light from any one of a plurality of detection emitters positioned about said prism and of reflecting the light toward said photomultiplier tube; and

a plurality of detection carriers, each of said detection carriers comprising a fiber bundle comprising means for carrying light from one of the wells to one of said detection emitters.

10. The apparatus of claim 9, wherein said prism is selectively rotatable between a plurality of predetermined positions, said prism in each position comprising means for receiving light from one of said detection emitters and of reflecting the light toward said photomultiplier tube.

11. The apparatus of claim 9, further comprising a plurality of collimating lenses, each of said lenses being positioned between one of said detection emitters and said prism.

12. The apparatus of claim 9, further comprising a wavelength filter positioned between said photomultiplier tube and said prism.

13. The apparatus of claim 12, wherein said wavelength filter comprises a dichroic filter and a bandpass filter.

14. The apparatus of claim 9, further comprising a baffle positioned between said photomultiplier tube and said prism.

15. The apparatus of claim 9, further comprising:

an excitation light source comprising means for producing excitation light; and

a plurality of excitation carriers, each of said excitation carriers comprising a fiber bundle comprising means for carrying light from said excitation light source toward one of the wells.

16. The apparatus of claim 15, wherein substantially all of the excitation light produced by said excitation light source has a wavelength lying in a wavelength band about 30 nanometers wide.

18. The apparatus of claim 12, wherein substantially all of the excitation light produced by said excitation light source has a wavelength in the range from about 470 nanometers to about 500 nanometers.

17. An apparatus for detecting light in a predetermined wavelength band, the light being transmitted from a composition disposed in one of a plurality of sample wells, said apparatus comprising:

a single photomultiplier tube;

a prism comprising means for selectively receiving light from any one of a plurality of detection emitters positioned about said prism and of reflecting the light toward said photomultiplier tube;

a plurality of collimating lenses, each of said lenses being positioned between one of said detection emitters and said prism;

a plurality of detection carriers, each of said detection carriers comprising a fiber bundle comprising means for carrying light from one of the wells to one of said detection emitters;

an excitation light source comprising means for producing excitation light; and

a plurality of excitation carriers, each of said excitation carriers comprising a fiber bundle comprising means for carrying light from said excitation light source toward one of the wells;

wherein said prism is selectively rotatable between a plurality of predetermined positions, said prism in each position comprising means for receiving light from one of said detection emitters and of reflecting the light toward said photomultiplier tube.

19. The apparatus of claim 17, wherein said fiber bundle of one of said detection carriers is interleaved with said fiber bundle of one of said excitation carriers near one of the wells.

20. The apparatus of claim 17, further comprising a wavelength filter positioned between said photomultiplier tube and said prism, said wavelength filter allowing passage of light having a wavelength in the range from about 510 nanometers to about 550 nanometers.

21. The apparatus of claim 17, further comprising a stepping motor having a shaft secured to said prism for selectively rotating said prism between said plurality of predetermined positions.