Process for the synchronization of ovulation

• US 5,589,457 A
• Filed: 07/03/1995
• Issued: 12/31/1996
• Est. Priority Date: 07/03/1995
• Status: Expired due to Fees

• Current Assignee: AUSA International Incorporated
• Original Assignee: AUSA International Incorporated
• Inventors: Wiltbank, Milo, Pursley, James R.
• Primary Examiner(s): Spivack, Phyllis G.
• Application Number:
  US08/498,691
• Time in Patent Office: 547 Days
• Field of Search: 514/12, 514/573, 514/15
• US Class Current: 514/10.1
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 31/557 Eicosanoids, e.g. leukotrienes or prostaglandins
• CPC Class Codes: A61K 38/24 Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g. HCG; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]

Abstract

A method for synchronizing ovulation in cattle by sequential injection of hormones is disclosed. A hormone selected from the group consisting of gonadotropin releasing hormone (GnRH), luteinizing hormone (LH), or human chorionic gonadotropin (hCG) is administered to an open cow during an estrous cycle in order to stimulate follicle development. Prostaglandin F₂α (PGF₂α) is then administered to initiate corpus luteum regression about five to eight days after administration of the GnRH, LH or hCG. A second dose of GnRH, LH or hCG is then administered concomitantly with the PGF₂α injection or up to about three days after the PGF₂α injection. This second dose of hormone functions to stimulate the ovulation of a dominant follicle and the cow is then breed within one day of the administration of the second dose of hormone.

Claims

1. A method for synchronizing ovulation in cattle, the steps comprising:

(a) administering a first dose of a hormone selected from the group consisting of a gonadotropin releasing hormone (GnRH), a luteinizing hormone (LH) and a human chorionic gonadotropin (hCG), to an open cow during an estrous cycle in an amount effective to stimulate ovarian follicle development;

(b) subsequent to step (a) and after a period of time sufficient for development of a dominant follicle, administering a dose of prostaglandin F₂α

(PGF₂α

) in an amount effective to initiate corpus luteum regression;

(c) concomitantly with or within a period of 3 days subsequent to step (b), administering a second dose of a hormone selected from the group consisting of LH, hCG and GnRH to stimulate ovulation of said follicle; and

(d) concomitantly with or within one day prior to or subsequent to step (c), breeding said cow.

2. The method of claim 1, wherein said cow is a lactating cow.

3. The method of claim 2, wherein said dose of PGF₂α

is administered about five to eight days after the first dose of hormone in step (a).

4. The method of claim 3, wherein said dose of PGF₂α

is administered about seven days after the first dose of hormone in step (a).

5. The method of claim 3, wherein said second dose of hormone in step (c) is administered about 11/2 to 2 days after administration of said dose of PGF₂α

.

6. The method of claim 3, wherein said cow is bred within a period extending from eight hours before to eight hours after the administration of said second dose of hormone in step (c).

7. The method of claim 3, wherein said cow is bred about the same time as the administration of said second dose of hormone in step (c).

8. The method of claim 4, wherein said cow is bred within a period of about 16-20 hours after the administration of said second dose of hormone in step (c).

9. The method of claim 1, wherein the hormone administered in step (a) is GnRH.

10. The method of claim 9, wherein the hormone dose administered in step (c) is GnRH.

11. The method of claim 9, wherein the GnRH in step (a) is administered in an amount of less than 100 micrograms.

12. The method of claim 11, wherein the second dose of GnRH administered in step (c) is administered in an amount of less than 100 micrograms.

13. The method of claim 12, wherein said PGF₂α

dose is about 20-35 milligrams.

14. The method of claim 10, wherein said second dose of GnRH is administered in step (c) in an amount which is less than the first dose of the GnRH administered in step (a).

15. The method of claim 10, wherein at least one of the doses of GnRH is administered in an amount within the range of 10-40 micrograms.

16. The method of claim 15, wherein said second dose of GnRH in step (c) is administered in an amount which is less than the first dose of the GnRH administered in step (a).

17. The method of claim 1, wherein at least one of said doses of hormone administered in steps (a) and (c) is selected from the group consisting of LH and hCG.

18. The method of claim 17, wherein the other of said doses of hormone administered in steps (a) and (c) is selected from the group consisting of LH and hCG.

19. The method of claim 1, wherein said first and second doses of hormone administered in steps (a) and (c) are luteinizing hormone.

20. The method of claim 1, wherein said first and second doses of hormone administered in steps (a) and (c) are human chorionic gonadotropin.

21. A method for synchronizing ovulation of cattle, the steps comprising:

(a) selecting a group of cows comprising a plurality of open cows;

(b) administering a first dose of a hormone selected from the group consisting of a gonadotropin releasing hormone (GnRH), a luteinizing hormone (LH) and a human chorionic gonadotropin (hCG), to said cows during estrous cycles in an amount effective to stimulate ovarian follicle development;

(c) subsequent to step (b) and after a period of time sufficient for development of dominant follicles in said group of cows, administering prostaglandin F₂α

(PGF₂α

) to said selected group of cows to initiate corpus luteum regression;

(d) concomitantly with or within a period of 3 days subsequent to step (c), administering a second dose of hormone selected from the group consisting of GnRH, LH and (hCG) to stimulate ovulation of said dominate follicles; and

(e) concomitantly with step (d) or within one day of the administration of said dose of GnRH in step (d), breeding said group of cows.

22. The method of claim 21, wherein said cows are lactating cows.

23. The method of claim 22, wherein the hormone in step (b) is GnRH administered in an amount of less than 100 micrograms per dose.

24. The method of claim 23, wherein said hormone in step (d) is GnRH and the second doses of GnRH in step (d) are administered in amounts of less than 100 micrograms per dose.

25. The method of claim 24, wherein said second doses of GnRH in step (d) are administered in amounts which are less than the first doses of the GnRH administered in step (b).

26. The method of claim 24, wherein at least one of the doses of the GnRH is administered in an amount within the range of 10-40 milligrams.

27. The method of claim 21, wherein individual cows within said selected group are at different stages in their estrous cycles.

28. The method of claim 27, wherein said cows are lactating cows.

29. The method of claim 21, wherein said doses of PGF₂α

are administered about five to eight days after the first dose of hormone in step (a).

30. The method of claim 21, wherein said second dose of hormone in step (d) is administered about 11/2 to 2 days after administration of said dose of PGF₂α

.

31. The method of claim 21, wherein said cow is bred within a period extending from eight hours before to eight hours after the administration of said second dose of hormone in step (d).

32. The method of claim 21, wherein said cow is bred about the same time as the administration of said second dose of hormone in step (d).

33. The method of claim 21, wherein said cows are bred within a period of about one day after the administration of said second dose of hormone in step (d).

34. The method of claim 21, wherein the hormone administered in step (b) is GnRH.

35. The method of claim 34, wherein the hormone dose administered in step (d) is GnRH.

36. The method of claim 21, wherein said second doses of GnRH are administered in step (d) in amounts which are less than the first doses of the GnRH administered in step (b).

37. The method of claim 21, wherein at least one of said doses of hormone administered in steps (a) and (d) is selected from the group consisting of LH and hCG.

38. The method of claim 37, wherein the other of said doses of hormone administered in steps (a) and (d) is selected from the group consisting of LH and hCG.

39. The method of claim 21, wherein said first and second doses of hormone administered in steps (a) and (d) are luteinizing hormone.

40. The method of claim 21, wherein said first and second doses of hormone administered in steps (a) and (d) are human chorionic gonadotropin.