Expression of human protein C in mammary tissue of transgenic mammals

• US 5,589,604 A
• Filed: 05/23/1994
• Issued: 12/31/1996
• Est. Priority Date: 01/11/1991
• Status: Expired due to Term

• Current Assignee: The American Red Cross, Virginia Intellectual Property Division
• Original Assignee: The American Red Cross, Virginia Intellectual Property Division
• Inventors: Wilkins, Tracy D., Drohan, William N., Velander, William H., Johnson, John L.
• Primary Examiner(s): Crouch, Deborah
• Application Number:
  US08/247,484
• Time in Patent Office: 953 Days
• Field of Search: 800/2, 435/172.3
• US Class Current: 800/7
• CPC Class Codes: A01K 2207/15 Humanized animals
• CPC Class Codes: A01K 2217/00 Genetically modified animals
• CPC Class Codes: A01K 2217/05 Animals comprising random inserted nucleic acids (transgenic)
• CPC Class Codes: A01K 2227/105 Murine
• CPC Class Codes: A01K 2267/01 Animal expressing industrially exogenous proteins
• CPC Class Codes: A01K 67/0278 Knock-in vertebrates, e.g. humanised vertebrates
• CPC Class Codes: C07K 2319/02 containing a signal sequence
• CPC Class Codes: C12N 15/8509 for producing genetically modified animals, e.g. transgenic
• CPC Class Codes: C12N 9/6464 Protein C (3.4.21.69)
• CPC Class Codes: C12Y 304/21069 Protein C activated (3.4.21.69)

Abstract

Recombinant protein C characterized by a high percentage of active protein can be obtained in the milk of transgenic mammals that incorporate DNAs according to the present invention. Transgenic mammals of the present invention are produced by introducing into developing embryos DNA that encodes protein C, such that the DNA is stably incorporated in the DNA of germ line cells of the mature mammals and inherited in normal, mendelian fashion.

Claims

1. A transgenic non-human mammal that contains and expresses a human protein C DNA construct in the cells of its mammary gland, wherein the DNA construct consists of:

(a) a mammary gland promoter,

(b) a nucleotide sequence that encodes a signal peptide, wherein said signal peptide is effective in directing the secretion of an associated polypeptide into the milk of said transgenic non-human mammal, and wherein said signal peptide-encoding nucleotide sequence is operatively associated with said mammary gland promoter, and

(c) a nucleotide sequence encoding human protein C that is operatively associated with said signal peptide-encoding nucleotide sequence,

wherein human protein C is secreted into the milk of said transgenic non-human mammal, and when purified, said protein C has a specific activity more than about 80% of the specific activity of human protein C isolated from human plasma, as determined by an assay of protein C serine protease activity or anticoagulant activity, and

wherein said non-human mammal is selected from the group consisting of mouse, pig, sheep, goat and cattle.

2. The transgenic non-human mammal of claim 1, wherein said promoter is selected from the group consisting of a whey acidic protein promoter, a casein promoter, a lactalbumin promoter and a β

-lactoglobulin promoter.

3. The transgenic non-human mammal of claim 2, wherein promoter is a whey acidic protein promoter or a β

-lactoglobulin promoter.

4. The transgenic non-human mammal of claim 3, wherein said promoter is a whey acidic protein promoter.

5. The transgenic non-human mammal of claim 1, wherein said human protein C isolated from said transgenic non-human mammal has a specific activity that is about 80% to about 100% of the specific activity of human protein C isolated from human plasma.

6. The transgenic non-human mammal of claim 5, wherein said specific activity is determined by an activated partial thromboplastin clotting time assay.

7. The transgenic non-human mammal of claim 5, wherein said promoter is a whey acidic protein promoter or a β

-lactoglobulin promoter.

8. A process for the production of protein C, comprising the steps of:

(a) providing a transgenic non-human mammal that contains and expresses a human protein C DNA construct in the cells of its mammary gland, wherein the DNA construct consists of;

(i) a mammary gland promoter,

(ii) a nucleotide sequence that encodes a signal peptide, wherein said signal peptide is effective in directing the secretion of an associated polypeptide into the milk of said transgenic non-human mammal, and wherein said signal peptide-encoding nucleotide sequence is operatively associated with said mammary gland promoter, and

(iii) a nucleotide sequence encoding human protein C that is operatively associated with said signal peptide-encoding nucleotide sequence,

wherein human protein C is secreted into the milk of said transgenic non-human mammal, and when purified, said protein C has a specific activity more than about 80% of the specific activity of human protein C isolated from human plasma, as determined by an assay of protein C serine protease activity or anticoagulant activity, and

wherein said non-human mammal is selected from the group consisting of mouse, pig, sheep, goat and cattle,

(b) producing milk from said transgenic non-human mammal,

(c) collecting said milk, and

(d) isolating said protein C from said milk.

9. The process of claim 8, wherein said promoter is selected from the group consisting of a whey acidic protein promoter, a casein promoter, a lactalbumin promoter and a β

-lactoglobulin promoter.

10. The process of claim 9, wherein promoter is a whey acidic protein promoter or a β

-lactoglobulin promoter.

11. The process of claim 10, wherein said promoter is a whey acidic protein promoter.

12. The transgenic non-human mammal of claim 8, wherein said human protein C isolated from said transgenic non-human mammal has a specific activity that is about 80% to about 100% of the specific activity of human protein C isolated from human plasma.

13. The transgenic non-human mammal of claim 12, wherein said specific activity is determined by an activated partial thromboplastin clotting time assay.

14. The transgenic non-human mammal of claim 13, wherein said promoter is a whey acidic protein promoter or a β

-lactoglobulin promoter.

15. A transgenic non-human mammal that contains and expresses a human protein C DNA construct in the cells of its mammary gland, wherein the DNA construct consists of:

(a) a mammary gland promoter selected from the group consisting of a whey acidic protein promoter, a casein promoter, a lactalbumin promoter and a β

-lactoglobulin promoter,

(b) a nucleotide sequence that encodes a signal peptide, wherein said signal peptide is effective in directing the secretion of an associated polypeptide into the milk of said transgenic non-human mammal, and wherein said signal peptide-encoding nucleotide sequence is operatively associated with said mammary gland promoter, and

(c) a nucleotide sequence encoding human protein C that is operatively associated with said signal peptide-encoding nucleotide sequence,

wherein human protein C is secreted into the milk of said transgenic non-human mammal, and when purified, said protein C has a specific activity more than about 80% of the specific activity of human protein C isolated from human plasma, as determined by an assay of protein C serine protease activity or anticoagulant activity, and

wherein said non-human mammal is selected from the group consisting of mouse, pig, sheep, goat and cattle.

16. The transgenic non-human mammal of claim 15, wherein said human protein C isolated from said transgenic non-human mammal has a specific activity that is about 80% to about 100% of the specific activity of human protein C isolated from human plasma.

17. The transgenic non-human mammal of claim 16, wherein said specific activity is determined by an activated partial thromboplastin clotting time assay.

18. A process for the production of protein C, comprising the steps of:

(a) providing a transgenic non-human mammal that contains and expresses a human protein C DNA construct in the cells of its mammary gland, wherein the DNA construct consists of;

(i) a mammary gland promoter selected from the group consisting of a whey acidic protein promoter, a casein promoter, a lactalbumin promoter and a β

-lactoglobulin promoter,

(ii) a nucleotide sequence that encodes a signal peptide, wherein said signal peptide is effective in directing the secretion of an associated polypeptide into the milk of said transgenic non-human mammal, and wherein said signal peptide-encoding nucleotide sequence is operatively associated with said mammary gland promoter, and

(iii) a nucleotide sequence encoding human protein C that is operatively associated with said signal peptide-encoding nucleotide sequence,

wherein human protein C is secreted into the milk of said transgenic non-human mammal, and when purified, said protein C has a specific activity more than about 80% of the specific activity of human protein C isolated from human plasma, as determined by an assay of protein C serine protease activity or anticoagulant activity, and

wherein said non-human mammal is selected from the group consisting of mouse, pig, sheep, goat and cattle,

(b) producing milk from said transgenic non-human mammal,

(c) collecting said milk, and

(d) isolating said protein C from said milk.

19. The transgenic non-human mammal of claim 18, wherein said human protein C isolated from said transgenic non-human mammal has a specific activity that is about 80% to about 100% of the specific activity of human protein C isolated from human plasma.

20. The transgenic non-human mammal of claim 19, wherein said specific activity is determined by an activated partial thromboplastin clotting time assay.