Spectrophotometric method and apparatus for the characterization of blood and blood types

• US 5,589,932 A
• Filed: 02/08/1995
• Issued: 12/31/1996
• Est. Priority Date: 02/08/1995
• Status: Expired due to Term

• Current Assignee: University of South Florida (State University System of Florida)
• Original Assignee: University of South Florida (State University System of Florida)
• Inventors: Bayona, Manuel, Garcia-Rubio, Luis H., Potter, Robert, Leparc, German
• Primary Examiner(s): Gonzalez, Frank
• Assistant Examiner(s): VIERRA EISENBERG, JASON D
• Application Number:
  US08/385,717
• Time in Patent Office: 692 Days
• Field of Search: 128/633, 364/413.07, 364/413.08, 356/39
• US Class Current: 356/39
• CPC Class Codes: G01N 21/31 Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry G01N21/72 takes precedence
• CPC Class Codes: G01N 21/49 within a body or fluid
• CPC Class Codes: G01N 33/80 involving blood groups or blood types or red blood cells white blood cells G01N33/56972

Abstract

A method and apparatus for determining the type of a blood sample are provided wherein a turbidity spectrum of the blood sample is collected over a predetermined wavelength range, from which is calculated an extinction spectrum. This extinction spectrum is then compared with a set of control spectra collected from control blood samples having known blood types, from which the type of the blood sample can be determined. A further method is provided for for detecting the presence of a substance in a bodily fluid sample, the substance having a size in the range of generally 0.5 to 20 μm. Exemplary substances that could be of interest to detect include, but are not limited to, hemoglobin, bilirubin, red blood cell antigens, microorganisms, and viruses. This embodiment includes the additional step of deconvoluting the extinction spectrum to obtain a particle size distribution for comparison with a database of control samples.

Claims

1. A method for determining the type of a blood sample, the method comprising the steps of:

collecting a turbidity spectrum of the blood sample over a predetermined wavelength range;

calculating from the turbidity spectrum an extinction spectrum for the blood sample;

comparing the extinction spectrum with a set of control spectra collected from control blood samples, each control sample having a known blood type; and

determining from the comparison the type of the blood sample.

2. The method for determining the type of a blood sample recited in claim 1, wherein the calculating step further comprises the step of normalizing intensity effects due to a concentration of red blood cells in the blood sample.

3. The method for determining the type of a blood sample recited in claim 1, wherein the predetermined wavelength range comprises the ultraviolet-to-visible wavelength range.

4. The method for determining the type of a blood sample recited in claim 3, wherein the ultraviolet-to-visible wavelength range comprises a wavelength range of 180 to 820 nm.

5. The method for determining the type of a blood sample recited in claim 4, wherein the collecting step further comprises collecting the turbidity spectrum with a 2 nm resolution.

6. The method for determining the type of a blood sample recited in claim 1, wherein the collecting step comprises collecting the turbidity spectrum with a spectrophotometer.

7. The method for determining the type of a blood sample recited in claim 6, further comprising the step, prior to the collecting step, of diluting the blood sample sufficiently to reach a linear range of the spectrophotometer.

8. An on-line method for spectrophotometrically determining the type of a whole blood sample, the method comprising the steps of:

diluting the blood sample with phosphate buffered saline to reach a red blood cell concentration in the range of 3900-4100 per milliliter and an optical density of 0.1 to 1.0 absorbance units in order to reach a linear range of the spectrophotometer;

collecting a turbidity spectrum of the blood sample with the spectrophotometer over a wavelength range of 180-820 nm with a resolution of 2 nm;

calculating from the turbidity spectrum an extinction spectrum for the blood sample, the calculation including a normalization of intensity effects due to the red blood cell concentration;

comparing the extinction spectrum with a set of control spectra collected from control blood samples, each control sample having a known blood type; and

determining from the comparison the type of the blood sample.

9. An apparatus for determining the type of a blood sample, the apparatus comprising:

means for collecting a turbidity spectrum of the blood sample over a predetermined wavelength range;

means for calculating from the turbidity spectrum an extinction spectrum for the blood sample;

means for comparing the extinction spectrum with a set of control spectra collected from control blood samples, each control sample having a known blood type; and

means for determining from the comparison the type of the blood sample.

10. The apparatus recited in claim 9, wherein the collecting means comprises a spectrophotometer.

11. The apparatus recited in claim 10, wherein the predetermined wavelength range comprises 180 to 800 nm.

12. The apparatus recited in claim 11, wherein the the spectrophotometer has sufficient sensitivity to collect the turbidity spectrum with a resolution of 2 nm.

13. The apparatus recited in claim 11, further comprising means for diluting the blood sample for reaching a linear range of the spectrophotometer.

14. The apparatus recited in claim 10, wherein the calculating means comprises a computer adapted to receive the tubidity spectrum from the spectrophotometer and software means for calculating an extinction spectrum from the turbidity spectrum resident in the computer.