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Method, test element and test kit for semi-quantitative detection of target nucleic acid

  • US 5,591,580 A
  • Filed: 03/31/1994
  • Issued: 01/07/1997
  • Est. Priority Date: 03/31/1994
  • Status: Expired due to Term
First Claim
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1. A method for the semi-quantitative determination of a target nucleic acid comprising:

  • A) contacting a target nucleic acid having a specific binding ligand bound thereto with a water-insoluble support having affixed thereon a multiplicity of detection deposits,each detection deposit comprising a mixture of water-insoluble first and second particles,said first particles having affixed thereto a capture probe which is specific to and hybridizable with said target nucleic acid, andsaid second particles being free of all capture probes,said multiplicity of detection deposits having a varying weight ratio of said first particles to said second particles, but all of said detection deposits having about the same total weight of polymeric particles and the same configuration,to capture said target nucleic acid strand on said detection deposits in proportion to the amount of said first particles in each detection deposit,B) contacting said captured target nucleic acid strand in said detection deposits with a receptor for said specific binding ligand, said receptor being labeled with a reporter molecule,to complex said reporter labeled receptor with said captured target nucleic acid strand and thereby capture said reporter labeled receptor on each of said detection deposits in proportion to the amount of captured ligand labeled target nucleic acid strand in each of said detection deposits, andC) detecting the reporter molecules on said detection deposits as a semi-quantitative determination of the presence of said target nucleic acid in a range of 0.08 to 1.25 pmol/ml nucleic acid.

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