Method, test element and test kit for semi-quantitative detection of target nucleic acid

• US 5,591,580 A
• Filed: 03/31/1994
• Issued: 01/07/1997
• Est. Priority Date: 03/31/1994
• Status: Expired due to Term

• Current Assignee: Clinical Diagnostic Systems Inc.
• Original Assignee: Johnson & Johnson Clinical Diagnostics Inc. (Johnson & Johnson)
• Inventors: Bergmeyer, Lynn, Cummins, Thomas J.
• Primary Examiner(s): Jones, W. Gary
• Assistant Examiner(s): Atzel, Amy
• Application Number:
  US08/221,818
• Time in Patent Office: 1,013 Days
• Field of Search: 435/6, 435/91.2, 422/57, 422/68.1
• US Class Current: 435/6.11
• CPC Class Codes: C12Q 1/6813 Hybridisation assays
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: C12Q 1/686 Polymerase chain reaction [PCR]

Abstract

A target nucleic acid can be detected in a semi-quantitative fashion by passing it over detection deposits in a test element. The detection deposits include particles affixed to the test element, some of which particles have capture probe attached thereto, and other particles having no capture probe. The deposits have varying amounts of capture probe so that the signal obtained when the target nucleic acid is captured thereon can be semi-quantitatively correlated to the amount of target nucleic acid in the specimen. This method of detection can be used in nucleic acid hybridization assays or following amplification methods, including polymerase chain reaction.

Claims

1. A method for the semi-quantitative determination of a target nucleic acid comprising:

A) contacting a target nucleic acid having a specific binding ligand bound thereto with a water-insoluble support having affixed thereon a multiplicity of detection deposits,

each detection deposit comprising a mixture of water-insoluble first and second particles,

said first particles having affixed thereto a capture probe which is specific to and hybridizable with said target nucleic acid, and

said second particles being free of all capture probes,

said multiplicity of detection deposits having a varying weight ratio of said first particles to said second particles, but all of said detection deposits having about the same total weight of polymeric particles and the same configuration,

to capture said target nucleic acid strand on said detection deposits in proportion to the amount of said first particles in each detection deposit,

B) contacting said captured target nucleic acid strand in said detection deposits with a receptor for said specific binding ligand, said receptor being labeled with a reporter molecule,

to complex said reporter labeled receptor with said captured target nucleic acid strand and thereby capture said reporter labeled receptor on each of said detection deposits in proportion to the amount of captured ligand labeled target nucleic acid strand in each of said detection deposits, and

C) detecting the reporter molecules on said detection deposits as a semi-quantitative determination of the presence of said target nucleic acid in a range of 0.08 to 1.25 pmol/ml nucleic acid.

2. The method of claim 1 wherein said specific binding ligand is biotin, and said receptor is streptavidin which is labeled with a radioisotope, chemiluminescent reagent or enzyme.

3. The method of claim 2 wherein said streptavidin is labeled with a peroxidase.

4. The method of claim 1 wherein said target nucleic acid having said ligand bound thereto is contacted with from two to ten detection deposits in step B, in order of the detection deposit having the least amount of said first particles to the detection deposit having the most amount of said first particles.

5. The method of claim 1 wherein said first and second particles have about the same average diameter.

6. The method of claim 1 wherein, simultaneously with contact of said target nucleic acid with said detection deposits, said target nucleic acid is hybridized with a detection probe conjugated with said specific binding ligand.

7. A method for the amplification and semi-quantitative determination of a target nucleic acid comprising:

A) amplifying opposing strands of a target nucleic acid with a DNA polymerase, more than one dNTP and a set of two primers specific to and hybridizable with said opposing strands, one of said primers being labeled with a specific binding ligand, thereby providing at least one amplified ligand labeled strand of said target nucleic acid,

B) contacting said amplified ligand labeled target nucleic acid strand with a water-insoluble support having affixed thereon a multiplicity of detection deposits,

each detection deposit comprising a mixture of water-insoluble first and second particles,

said first particles having affixed thereto a capture probe which is specific to and hybridizable with said ligand labeled target nucleic acid strand, and

said second particles being free of all capture probes,

said multiplicity of detection deposits having a varying weight ratio of said first particles to said second particles, but all of said detection deposits having about the same total weight of polymeric particles and the same configuration,

to capture said ligand labeled target nucleic acid strand on said detection deposits in proportion to the amount of said first particles in each detection deposit,

C) contacting said captured ligand labeled target nucleic acid strand in said detection deposits with a receptor for said specific binding ligand, said receptor being labeled with a reporter molecule,

to complex said reporter labeled receptor with said captured ligand labeled target nucleic acid strand and thereby capture said reporter labeled receptor on each of said detection deposits in proportion to the amount of captured ligand labeled target nucleic acid strand in each of said detection deposits, and

D) detecting the reporter molecules on said detection deposits as a semi-quantitative determination of the presence of said target nucleic acid in a range of 0.08 to 1.25 pmol/ml nucleic acid.

8. The method of claim 7 wherein said specific binding ligand label on said primer is biotin.

9. The method of claim 8 wherein amplification is carried out using a thermostable DNA polymerase, each of dATP, dCTP, dGTP and dTTP and a DNA polymerase cofactor, said receptor is streptavidin, and said reporter molecule is a peroxidase which is detected by contact with one or more reagents which provide a colorimetric signal in response to the reaction of peroxidase with a substrate.

10. The method of claim 7 wherein amplification is carried out with from 20 to 50 polymerase chain reaction cycles.

11. The method of claim 7 wherein said first and second particles have about the same average diameter, and said ligand labeled target nucleic acid is contacted with from two to ten detection deposits in step B, in order of the detection deposit having the least amount of said first particles to the detection deposit having the most amount of said first particles.

12. A test element comprising a water-insoluble support having affixed thereon a multiplicity of detection deposits,

each detection deposit comprising a mixture of water-insoluble first and second particles,

said first particles having affixed thereto a capture probe which is specific to and hybridizable with a ligand labeled target nucleic acid, and

said second particles being free of capture probes,

said multiplicity of detection deposits having a varying weight ratio of said first particles to said second particles, but all of said detection deposits having about the same total weight of polymeric particles and the same configuration.

13. The test element of claim 12 wherein said support is a polymeric membrane, filter paper, fibrous mat, resin-coated film or paper or uncoated film or paper.

14. The test element of claim 12 wherein said support is heat or ultrasonic sound sealable resin-coated paper or polymeric film which has been pretreated by corona discharge or by application of a resinous hydrophilic subbing layer.

15. The test element of claim 12 wherein each of said detection deposits comprises a polymeric adhesive present in an amount of up to about 20 weight % of the total dry weight of each of said detection deposits.

16. The test element of claim 12 having from two to ten detection deposits which are circular spots on said support.

17. A kit for the detection of a target nucleic acid labeled with a specific binding ligand comprising:

a) either an amplification reagent, or a reporter labeled receptor for said specific binding ligand, and

b) a test element comprising a water-insoluble support having affixed thereon a multiplicity of detection deposits,

each detection deposit comprising a mixture of water-insoluble first and second particles,

said first particles having affixed thereto a capture probe which is specific to and hybridizable with a ligand labeled target nucleic acid, and

said second particles being free of capture probes,

said multiplicity of detection deposits having a varying weight ratio of said first particles to said second particles, but all of said detection deposits having about the same total weight of polymeric particles and the same configuration.

18. The test kit of claim 17 comprising one or more amplification reagents, and wherein said test element has from two to ten detection deposits which are circular spots on said support, and said first and second particles having about the same average diameter.

19. The test kit of claim 18 comprising a thermostable DNA polymerase, a cofactor for said DNA polymerase, each of dATP, dCTP, dGTP and dTTP, streptavidin labeled with peroxidase, and at least one primer which is specific to and hybridizable with said target nucleic acid, said primer being labeled with biotin.

20. The test kit of claim 17 wherein said support is a heat or ultrasonic sound sealable material which has been pretreated by corona discharge or by application of a hydrophilic subbing layer, and

each of said detection deposits comprising a polymeric adhesive which is present in an amount of up to about 20 weight % of the total dry weight of each of said detection deposits.