System for detecting bacterial growth in a plurality of culture vials

• US 5,595,708 A
• Filed: 11/18/1994
• Issued: 01/21/1997
• Est. Priority Date: 08/27/1993
• Status: Expired due to Term

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Berndt, Klaus W.
• Primary Examiner(s): Housel, James C.
• Assistant Examiner(s): FREED, RACHEL
• Application Number:
  US08/341,825
• Time in Patent Office: 795 Days
• Field of Search: 422/63, 422/67, 422/68.1, 422/82.057, 422/82.09, 436/47, 436/163, 436/164, 436/172, 356/337, 356/432, 356/436, 435/291, 435/240.2, 435/4
• US Class Current: 422/82.06
• CPC Class Codes: C12M 41/36 of biomass, e.g. colony counters or by turbidity measurements electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404
• CPC Class Codes: C12M 41/46 of cellular or enzymatic activity or functionality, e.g. cell viability
• CPC Class Codes: C12Q 1/04 Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor C12Q1/6897 takes precedence
• CPC Class Codes: G01N 2021/7786 Fluorescence
• CPC Class Codes: G01N 2035/00752 bar codes
• CPC Class Codes: G01N 21/01 Arrangements or apparatus for facilitating the optical investigation
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/6408 with measurement of decay time, time resolved fluorescence
• CPC Class Codes: G01N 2201/0438 Linear motion, sequential
• CPC Class Codes: G01N 2201/101 Scanning measuring head
• CPC Class Codes: G01N 35/028 having reaction cells in the form of microtitration plates
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus

Abstract

A system for detecting the presence of bacterial growth in a plurality of sample vials incorporates a single test station moveable along each of the plurality of sample vials. In one embodiment, the sensor station is movably mounted on a rod, and that rod is movably mounted on a pair of spaced rods. The rod which carries the test station may move along the spaced rods to change the location of the test station in a first dimension and the test station is moveable along its rod to change location in a second dimension. In this way, the test station may be moved through two dimensions to move serially to the location of each of the plurality of sample vials. In another aspect of this invention, a bar code is associated with each of the sample vials, and the test station makes a reading of that bar code concurrent with a determination being made as to whether there is any bacterial growth in the sample vial. In this way, it is ensured that the results of the evaluation of whether bacterial growth is ongoing will be associated with the proper sample vial. In a third aspect of this invention, the sample vial incorporates a plurality of distinct types of bacterial sensors. Thus, the advantages of each of several types of bacterial sensors may be incorporated into a single sample vial.

Claims

1. A system for detecting bacterial growth in a plurality of sample vials, said system comprising:

a housing;

a plurality of sample vials containing a sensor;

a mount structure capable of mounting said plurality of sample vials within said housing;

a frame movably mounted in said housing;

a portion of a bacterial growth detection station mounted on said frame for gathering a first and second type of information to make a determination of whether each of said plurality of sample vials is experiencing bacterial growth, wherein said second type of information is provided by said sensor in a particular sample vial and said bacterial growth detection station portion on said frame includes a first light radiation source at a first location and a detector at a second location spaced from said first location by a first distance;

said mount structure includes a pair of light stubs spaced by a distance which approximates said first distance and positioned adjacent to each of said vials, one of said pair of stubs receiving said first light radiation and the other of said pair of stubs directing a reflected radiation out of said particular sample vial and into said detector to provide said first type of information; and

a controller for moving said frame to position said bacterial growth detection station portion adjacent to said particular sample vial mounted in said mount structure.

2. A system as recited in claim 1, wherein said frame is driven to move in two dimensions along an x and a y axis, and said mount structure mounts said plurality of sample vials along the x and the y axis.

3. A system as recited in claim 2, wherein said moving frame is mounted on a pair of spaced rods, guides are mounted on said pair of rods, a second rod extends between said guides, said guides being moveable on said pair of rods, and said bacterial growth detection station portion being moveable along said second rod such that said test station portion may move through two dimensions.

4. A system as recited in claim 1, wherein said movably mounted frame also carries a bar code reading apparatus for reading bar code information from the sample vials.

5. A system as recited in claim 4, wherein said detector, and said bar code reading apparatus are provided by a single detector.

6. A system as recited in claim 5, wherein said single detector is a CCD camera.

7. A system as recited in claim 4, wherein said movably mounted frame carries a second detector for providing said bar code reading function.

8. A system as recited in claim 1, wherein said pair of light stubs is provided by a first stub having an angled surface facing said particular sample vial to which it feeds said first fight radiation, and a second stub having an inclined surface facing said particular sample vial from which it receives said reflected radiation.