Oligonucleotide probe and primers specific to bovine or porcine male genomic DNA

• US 5,596,089 A
• Filed: 02/14/1994
• Issued: 01/21/1997
• Est. Priority Date: 02/14/1994
• Status: Expired due to Fees

• Current Assignee: Universite De Montreal, L'
• Original Assignee: Universite De Montreal, L'
• Inventors: Houde, Alain, Silversides, David W., Daneau, Isabelle M.
• Primary Examiner(s): Zitomer, Stephanie W.
• Assistant Examiner(s): Marschel, Ardin H.
• Application Number:
  US08/196,016
• Time in Patent Office: 1,072 Days
• Field of Search: 435/6, 435/91.2, 435/172.3, 435/810, 436/501, 536/23.1, 536/24.1, 536/24.3-24.33, 935/77, 935/78, 935/88
• US Class Current: 536/24.3
• CPC Class Codes: A01K 2217/05 Animals comprising random inserted nucleic acids (transgenic)
• CPC Class Codes: A01K 2217/075 inducing loss of function, i.e. knock out
• CPC Class Codes: C07K 14/47 from mammals
• CPC Class Codes: C12Q 1/6879 for sex determination
• CPC Class Codes: C12Q 2600/158 Expression markers
• CPC Class Codes: Y10S 435/81 Packaged device or kit

Abstract

The present invention relates to novel bovine (SEQ ID NO:1) and porcine (SEQ ID NO:2) genomic sequences for the SRY gene along with oligonucleotide primers. The present invention also relates to a method of sexing bovine or porcine tissue by descriminating PCR products obtained by amplification of specific DNA or cDNA sequences of bovine or porcine tissue which is used as a DNA template and wherein two pairs of DNA primers are used for the PCR. The present invention also relates to a method for the genetic manipulation or selection of sexual phenotype in domesticated animals, which comprises using transgenes composed of SRY sequences to cause and control the expression of genetic ablation sequences and genetic switching sequences in undifferentiated and developing gonadal tissues of both XX and XY animals.

Claims

1. An oligonucleotide probe for detection of bovine or porcine male genomic DNA, which comprises the nucleotide sequences selected from the group consisting of SEQ ID NO:

1 and SEQ ID NO;

2, or fragments thereof which comprise sequences outside of the HMG box, wherein no notable homology is seen between species and whereby, the oligonucleotide probe either exclusively or predominantly hybridizes to sequences outside of said HMG box.

2. An oligonucleotide primer for detection of bovine or porcine male genomic DNA, which comprises a DNA sequence selected from the group consisting of:

GCCGCGAGATGGCCATTCTTCCAGGAGGC SEQ ID NO;

3;

GGGAATTCAGATCAGCAAGCAGCTGGGA SEQ ID NO;

5;

ACAGTCATAGCGCAAATGATCAGTG SEQ ID NO;

6;

GAGAACATGTTAGGGAGAGCAGCCA SEQ ID NO;

7;

GGGTTGCATAGTATTGAAGAGTCTGC SEQ ID NO;

8;

GAAGCGATTTCTGTGGCCTCTTGG SEQ ID NO;

9;

GAATTCAGCCAATGTTACCTTATTGTGGC SEQ ID NO;

10;

GATTCAGCTGCTGTGATGCTCT SEQ ID NO;

11;

TATGCAATCGATGCATGT SEQ ID NO;

12;

ATAGCCCGGGTATTTGTCTC SEQ ID NO;

13;

GAATTCTAGTAGTCTCTGTGCCTCC SEQ ID NO;

14;

GCGAGAGTAGTTGTGC SEQ ID NO;

15;

CTACTCCCCAACCGTCA SEQ ID NO;

16;

TCTTCCTTGTGCACAGACAG SEQ ID NO;

17;

TTATTGTCGCCCAGGCTTGT SEQ ID NO;

18;

CACACTGATCATTTGCGC SEQ ID NO;

19;

AGGGTTAGTGATAGCAT SEQ ID NO;

20;

AGAAACAAGAATGACGA SEQ ID NO;

21;

GTTAGCAGAGCCTCCAG SEQ ID NO;

22;

AAGTCACTCACAGCCCA SEQ ID NO;

23;

CATGGGTCGCTTGACAC SEQ ID NO;

24;

GCTATGCTAAGGATTAT SEQ ID NO;

25;

GACAATTCATAGCTCAAACGATGGAC SEQ ID NO;

26;

ACTAGAGGAAGTGGTAGAGAGAGTG SEQ ID NO;

27;

ACTTGGCTGCATAGTACTGCCGCC SEQ ID NO;

28; and

GGAAGCAAATTCTGTGCCCTCTCTC SEQ ID NO;

29.