Device and a method for the electrophoretic separation of fluid substance mixtures
First Claim
1. A device for the electrophoretic separation of complex fluid substance mixtures, comprising a channel system for carrier media, an injection device for the injection into a first carrier medium of a substance mixture to be separated, and a first separating path for the separation of the substance mixture in an electric field that is applied in the first carrier medium along the first separating path, wherein for the further separation of the substance mixture there is provided at least one second separating path having a second carrier medium, which second carrier medium is different from the first carrier medium, in which a further electric field is applied along the second separating path, which second separating path is inclined at an angle with respect to the first separating path and, downstream of and at a distance from the injection device for the substance mixture to be separated, extends in such a manner that the region of intersection of the first separating path with the second separating path forms a second injection device for injecting the partially separated substance mixture into the second carrier medium wherein the injection volume of the second injection device is the volume of the second separating path between a point of entry of the first separating path into the second separating path and an opening in the second separating path which leads to an outflow for the first carrier medium.
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Accused Products
Abstract
A device for the electrophoretic separation of complex fluid substance mixtures comprises a channel system (21, 2, 22) for a carrier medium (C), an injection device (3) for the injection into the carrier medium (C) of a substance mixture (S) to be separated, and a separating path (2) for the separation of the substance mixture (S) in an electric field applied along the separating path (2). Downstream of the injection device (3) for the substance mixture (S) to be separated and at a distance therefrom there is provided a second separating path (4) for the further separation of the substance mixture (S) in an electric field applied along the second separating path (4). The second separating path (4) is inclined at an angle (α) with respect to the first separating path (2). The region of intersection of the first (2) and second (4) separating paths forms a second injection device (5) for injecting the partially separated substance mixture (S) into a second carrier medium (E). The fluid substance mixture (S) is injected into the carrier medium (C) in the first injection device (3) and is then separated in the electric field that is applied along the first separating path (2). The partially separated substance mixture (S) is then injected into the second carrier medium (E) downstream of the first separating path (2) and further separated into its components in the electric field applied along the second separating path (4).
302 Citations
22 Claims
- 1. A device for the electrophoretic separation of complex fluid substance mixtures, comprising a channel system for carrier media, an injection device for the injection into a first carrier medium of a substance mixture to be separated, and a first separating path for the separation of the substance mixture in an electric field that is applied in the first carrier medium along the first separating path, wherein for the further separation of the substance mixture there is provided at least one second separating path having a second carrier medium, which second carrier medium is different from the first carrier medium, in which a further electric field is applied along the second separating path, which second separating path is inclined at an angle with respect to the first separating path and, downstream of and at a distance from the injection device for the substance mixture to be separated, extends in such a manner that the region of intersection of the first separating path with the second separating path forms a second injection device for injecting the partially separated substance mixture into the second carrier medium wherein the injection volume of the second injection device is the volume of the second separating path between a point of entry of the first separating path into the second separating path and an opening in the second separating path which leads to an outflow for the first carrier medium.
- 15. A method for the electrophoretic separation of complex fluid substance mixtures along separating paths, in which a complex substance mixture is injected into a first carrier medium upstream of a first separating path and is then separated in an electric field that is applied along the first separating path, wherein the partially separated substance mixture is injected into a second separating path having a second carrier medium, which second carrier medium is different from the first carrier medium, and which second separating path intersects with the first separating path and inclines at an angle with respect to the first separating path such that the region of intersection of the first separating path with the second separating path forms an injection device having an injection volume which is the volume of the second separating path between a point of entry of the first separating path into the second separating path and an opening in the second separating path which leads to an outflow for the first carrier medium, and wherein the substance mixture is further separated in an electric field applied in the second carrier medium along the second separating path.
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22. A method for the electrophoretic separation of complex fluid substance mixtures along separating paths, in which a complex substance mixture is injected into a first carrier medium upstream of a first separating path and is then isoelectrically focused in an electric field that is applied along the first separating path, wherein the isoelectrically focused substance mixture is injected into a second separating path having a second carrier medium, which second carrier medium is different from the first carrier medium, and which second separating path intersects with the first separating path and inclines at an angle with respect to the first separating path such that the region of intersection of the first separating oath with the second separating path forms an injection device having an injection volume which is the volume of the second separating path between a point of entry of the first separating path into the second separating path and an opening in the second separating path which leads to an outflow for the first carrier medium, and wherein the substance mixture is separated in an electric field applied in the second carrier medium along the second separating path.
Specification