Allelic ladders for short tandem repeat loci

• US 5,599,666 A
• Filed: 03/28/1994
• Issued: 02/04/1997
• Est. Priority Date: 03/28/1994
• Status: Expired due to Fees

• Current Assignee: Promega Corporation
• Original Assignee: Promega Corporation
• Inventors: Schumm, James W., Puers, Christoph
• Primary Examiner(s): Leguyader, John L.
• Assistant Examiner(s): PRIEBE, SCOTT DAVID
• Application Number:
  US08/219,633
• Time in Patent Office: 1,044 Days
• Field of Search: 435/6, 536/23.1, 536/24.31
• US Class Current: 435/5
• CPC Class Codes: C12Q 1/6827 for detection of mutation or polymorphism
• CPC Class Codes: C12Q 1/6858 Allele-specific amplification
• CPC Class Codes: C12Q 1/6881 for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
• CPC Class Codes: C12Q 2525/151 repeat or repeated sequences, e.g. VNTR, microsatellite, concatemer
• CPC Class Codes: C12Q 2545/101 with an internal standard/control
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers

Abstract

The present invention is directed to an assay system, a kit and a process for detecting at least one short tandem repeat sequence from DNA at a specific locus utilizing an allelic ladder containing at least two short tandem repeat sequences of the same lengths as two or more known alleles for the locus.

Claims

1. An allelic ladder for evaluating short tandem repeat sequences at a specific locus wherein the locus and the minimum number of alleles in each ladder for each specific locus is selected from the group consisting of:

HUMCSF1PO containing at least 9 alleles, HUMF13A01 containing at least 12 alleles, HUMFESFPS containing at least 8 alleles, HUMLPL (LIPOL) containing at least 7 alleles, and HUMVWFA31 containing at least 7 alleles.

2. An allelic ladder specific for the STR locus HUMHPRTB (HPRT-1) containing at least 12 alleles.

3. A kit for analyzing short tandem repeat sequences from a DNA sample at a specific locus selected from the group consisting of HUMCSF1PO, HUMF13A01, HUMFESFPS, HUMLPL (LIPOL), HUMVWFA31, and HUMHPRTB (HPRT-1) comprising:

a container containing an allelic ladder specific for the locus, wherein the allelic ladder for HUMCSF1PO contains at least 9 alleles, the allelic ladder for HUMF13A01 contains at least 12 alleles, the allelic ladder for HUMFESFPS contains at least 8 alleles, the allelic ladder for HUMLPL (LIPOL) contains at least 7 alleles, the allelic ladder for HUMVWFA31 contains at least 7 alleles, and the allelic ladder for HUMHPRTB contains at least 12 alleles.

4. The kit of claim 3 further comprising additional containers either each containing one primer of a primer pair or containing a primer pair for said specific loci.